兔VX2肝癌模型的数字减影血管造影影像分析及改良肝动脉插管术  

Evaluation of digital subtraction angiography rabbit VX2 hepatic carcinoma model with modified hepatic artery catheterization

作　　者：刘鸿[1] 冯巧灵[2] 张玮[3] 谢拥军[4] 李超[5] 姜恒[2] 周恩亮[6] 

机构地区：[1]成都医学院临床医学系,成都610083 [2]成都医学院检验医学院,成都610083 [3]成都医学院第一附属医院整形烧伤外科,成都610083 [4]成都医学院基础医学院人体解剖学实验室,成都610083 [5]四川省肿瘤医院头颈外科,成都610041 [6]苍山县人民医院骨科,山东临沂277700

出　　处：《北京大学学报（医学版）》2013年第4期649-653,共5页Journal of Peking University:Health Sciences

基　　金：国家实用新型专利项目(ZL201120120345.2);四川省卫生厅科研基金项目(090538);成都医学院自然科学基金项目(CYZ09-004)资助~~

摘　　要：目的:探讨不同植瘤方式制备兔肝癌模型的成瘤率,并评估新型插管装置及改良术在实验研究中的应用。方法:将30只家兔随机均分为2组:Ⅰ组前期直视下开腹行肝穿刺瘤细胞悬液注入法制备兔VC2肝癌模型,后期数字减影血管造影(digital subtration angiography,DSA)定位下,以Seldinger穿刺插管术经股动脉行兔选择性肝动脉造影;Ⅱ组前期行直视下开腹肝穿刺瘤组织块注入法制备兔VX2肝癌模型,后期DSA定位下,循新型插管装置以改良插管法经股动脉行兔选择性肝动脉造影。结合DSA及病理检查分析肿瘤的影像学和组织学特征,并对两组兔肝癌模型复制的成功率,以及插管操作时间、术后效果进行比较和评价。结果:Ⅰ组与Ⅱ组兔肝癌模型制备的成功率分别为60.0%和93.3%,一次性插管的成功率分别为66.7%和92.8%,行股动脉插管所用时间分别为(35.6±5.8)min和(27.4±5.3)min,行肝动脉插管造影术后不良反应的发生率分别为42.8%和14.3%,上述指标两组相比,差异均有统计学意义(P均<0.05)。结论:直视下开腹行肝穿刺瘤组织块种植法与瘤细胞悬液种植法相比,成瘤效果更佳。新型插管装置及改良术较用常规装置以Seldinger法经股动脉行肝动脉造影,大幅提高了实验家兔肝动脉插管的质量和效率。Objective： To modify the success rate of establishing VX2 transplanted tumor model with different methods in rabbits,and access new typed modification and improved technique in catheterization.Methods： In the study,30 rabbits were randomly divided into 2 groups.In prophase,tumor cell suspension was implanted in group Ⅰ,while tumor tissue particles were implanted into liver under direct vision,to establish VX2 transplanted tumor model.The rabbits were catheterized from femoral artery to selective hepatic artery under DSA,by using conventional modification with Seldinger technique in group I and by using new typed modification with improved technique in group Ⅱ.The imaging and histological features of VX2 tumor were evaluated by combining pathology and DSA,then the success rate,operation time and postoperative complications were compared and evaluated.Results： The success rates of the liver tumor model were 60.0% and 93.3%;the disposable success rates of catheterization were 66.7% and 92.8%;the operation time of catheterization were（35.6±5.8） min and（27.4±5.3） min;the incidence rates of adverse reaction were 22.5% and 18.0%;the differences between the two groups in the experimental rabbits were significant（P＆lt;0.05） statistically.Conclusion： The efficiency of tumor tissue particles implanted is better than that of tumor cell suspension implanted in establishing VX2 transplanted tumor model under direct vision.The cathetenzation quality and outcomes of new typed modification by improved technique,from femoral artery to selective hepatic artery,is superior to those of conventional modification with Seldinger technique.

关 键 词：肝肿瘤 化学栓塞 治疗性 肝动脉 血管造影术 数字减影 兔 

分 类 号：R735.7[医药卫生—肿瘤]