胚胎发育早期神经上皮的显微移植技巧及应用  

Skill and application of neuroepithelial transplantation in early development of amniote embryo

作　　者：白仲添[1,2] 严俊 孟文勃 张磊 周文策[1] 李汛 

机构地区：[1]兰州大学第一医院普外二科,甘肃省肝胆胰外科研究所,兰州大学临床医学院肿瘤防治中心,甘肃兰州730000 [2]Department of Neuroanatomy, Institute of Anatomy, University of Bonn. Germany 53115 Bonn

出　　处：《中国比较医学杂志》2013年第8期48-51,66,共5页Chinese Journal of Comparative Medicine

基　　金：兰州大学中央高校基本科研业务费专项资金(编号:lzujbky-2012-167);甘肃省自然科学研究基金计划(1208RJZA219);中科院“西部之光”项目(编号:[2009]236);甘肃省卫生行业科研计划项目(编号:GSWST 2011-04)

摘　　要：脊椎动物胚胎发育早期神经上皮的显微移植在运动神经元的轴突寻路、神经嵴细胞的迁移等研究中起非常重要的作用。本文以鸡胚、鸡胚-鹌鹑胚胎嵌合体为研究对象,摈弃了传统的神经上皮显微移植带来的移植失败、胚胎死亡及难以操作等缺点,总结近年来国外显微移植技术优缺点及作者的反复实验,从胚胎的预处理、显微操作针的制备,到神经上皮的显微移植等多方面详细探讨成功进行显微移植的方法和经验。为神经发育生物学,尤其是早期胚胎发育的神经干细胞迁移及轴突寻路的研究提供方法上的基础。Neuroepithelial transplantation plays a critical role in axon pathfinding of motor neurons and migration of neural crest cells during early development of vertebrate embryo. In the present study animal models of chick embryo and chick-quail chimera were used to improve the mature methods of neuroepithelial transplantation. Many drawbacks such as embryo death, poor repeatability and cumbersome processes, etc. exist in traditional transplantation. Hence, we summarized the advantages and drawbacks of microsurgical transplantation reported in the literature, and developed our method based on our own experiments and experience. Embryo pre-treatment, microneedle preparation and some details were described in this paper to highlight how to achieve a successful micro-transplantation. The protocol will dedicate to developmental neurobiology research, such as migration of neural stem cells and axon pathfinding in the early embryonic development.

关 键 词：神经上皮 显微移植 嵌合体 轴突寻路 

分 类 号：R33[医药卫生—人体生理学]