葡萄籽原花青素抑制Hep-2细胞并诱导凋亡的实验研究  

Effects of grape seed procyanidin extract on proliferation and apoptosis of human laryngeal carcinoma cell line Hep-2

作　　者：赵岩[1] 刘丽 何群[1] 刘玉林[1] 于健[1] 王启威[1] 秦晓威[1] 韩国钧[1] 

机构地区：[1]哈尔滨医科大学附属第一医院耳鼻咽喉头颈外科,黑龙江哈尔滨150001 [2]望奎县中医院耳鼻咽喉科,黑龙江望奎152100

出　　处：《哈尔滨医科大学学报》2013年第4期308-311,共4页Journal of Harbin Medical University

基　　金：黑龙江省卫生厅科研课题(2011-010);哈医大一院科研基金(2011)

摘　　要：目的探讨葡萄籽提取物原花青素(grape seed procyanidin extract,GSPE)对人喉表皮样癌Hep-2细胞增殖和凋亡的影响。方法 Hep-2细胞与25、50、100和200μg/mL的GSPE共同孵育48 h后,采用噻唑蓝(MTT)法检测细胞增殖抑制率,流式细胞术分析细胞凋亡情况;用Western blot法检测不同浓度的GSPE作用于肿瘤细胞时,凋亡相关蛋白Bcl-2,bax表达情况。结果不同浓度(25、50、100和200μg/mL)GSPE对Hep-2细胞的增殖抑制率分别为(20.0±2.3)%、(33.6±2.7)%、(52.7±2.1)%和(76.6±2.3)%;凋亡率分别为(4.99±0.02)%、(26.49±2.49)%、(46.09±1.18)%和(58.77±2.38)%;细胞增殖抑制率和凋亡率均随GSPE浓度的升高而增高(P<0.05);Western blot检测药物处理细胞后Bcl-2表达水平下调,而Bax表达水平明显上调。结论 GSPE在体外可呈浓度依赖性抑制人喉表皮样癌Hep-2细胞增殖并促进其凋亡。Objective To investigate the effects of grape seed procyanidin extract （GSPE）on proliferation and apoptosis of human laryngeal carcinoma cell line Hep-2. Methods Hep-2 cells were cultured with different concentrations of GSPE （25, 50, 100 or 200 μg/mL）for 48 hours, inhibition of cell proliferation in vitro was measured by MTT assay, and apoptosis was detected by flow cytometry ; The expression of the Bcl-2, bax which associated with cell apoptosis were measured by Western blot. Results The inhibition rates were （20. 0± 2. 3 ）%, （33.6 ±2. 7）%, （ 52. 7 ±2. 1 ）% and （76. 6 ±2. 3）%, respectively, while the apoptotic rates were （4.99 ±0.02）%, （26.49±2.49）%, （46.09±1.18）% and （58.77±2.38）%. Both the inhibition rate and the apoptotic rate elevated with the increasing GSPE concentration （ P 〈 0.05 ） ; Western blot also showed that GSPE down-regulated Bcl-2 and up-regulated Bax. Conclusion GSPE inhibits the growth of Hep-2 cells and induces apoptosis in vitro in a concentration-dependent manner.

关 键 词：喉肿瘤 葡萄籽提取物原花青素 增殖 凋亡 

分 类 号：R739.65[医药卫生—肿瘤]