诱导小鼠胎肝干细胞向类心肌细胞的分化  

Mouse embryonic hepatic stem cells differentiate into cardiomyocyte-like cells

作　　者：吴必刚 常静[2] 张晓刚[2] 

机构地区：[1]重庆市铜梁县人民医院,重庆市402560 [2]重庆医科大学附属第一医院心内科,重庆市400016

出　　处：《中国组织工程研究》2013年第32期5813-5818,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:近年来胚胎肝干细胞备受关注,该类细胞是否具有向类心肌细胞方向分化的潜能及相关的诱导分化条件,目前报道极少。目的:探讨体外环境下化学试剂诱导小鼠胎肝干细胞向类心肌细胞方向分化的合适条件。方法:二甲基亚砜联合5-氮杂胞苷以不同的浓度和时间,作用于13.5d胎龄的昆明小鼠胎肝干细胞,观察诱导分化效果。结果与结论:体外环境下,0.8%二甲基亚砜+5μmol/L5-氮杂胞苷能诱导小鼠胎肝干细胞表达心肌细胞特异性标志物,而增加诱导剂浓度及延长时间不能提高诱导效率。BACKGROUND： In recent years, embryonic hepatic stem cells have attracted more attention, but there are few reports on the potential of embryonic hepatic stem cells to differentiate into cardiomyocyte-like cells as well as the related differentiation conditions. OBJECTIVE： To investigate the moderate condition to induce mice embryonic hepatic stem cells to differentiate into cardiomyocyte-like cells in vitro with chemical reagents. METHODS： Dimethylsulfoxide in combination with 5-azacytidine with different concentrations and time were used to induce the embryonic hepatic stem cells of 13.5 days mice and to observe the differentiation effect. RESULTS AND CONCLUSION： Under in vitro conditions, 0.8% dimethylsulfoxide＋5 μmol/L 5-azacytidine could induce the mouse embryonic hepatic stem cells to express the specific markers of myocardial cells, while increasing the concentration of the inducer and extending the induction time could not improve the induction efficacy.

关 键 词：干细胞 胚胎干细胞 胎肝干细胞 二甲基亚砜 5-氮杂胞苷 诱导 分化 类心肌细胞 Α-ACTIN 肌钙蛋白T 干细胞图片文章 

分 类 号：R394.2[医药卫生—医学遗传学]