透骨消痛胶囊促进成骨细胞的增殖和分化  

Tougu Xiaotong capsule promotes the proliferation and differentiation of osteoblasts

作　　者：黄云梅[1] 陈文列[1] 林如辉[1] 黄美雅[1] 李钻芳[1] 廖乃顺[1] 刘献祥[1] 

机构地区：[1]福建中医药大学,福建中西医结合研究院,福建省中西医结合老年性疾病重点实验室,国家中医药三级科研实验室-中药药理(细胞结构与功能)实验室,福建省福州市350122

出　　处：《中国组织工程研究》2013年第33期5923-5928,共6页Chinese Journal of Tissue Engineering Research

基　　金：福建省自然科学基金项目(2010J01200);陈可冀中西医结合发展基金项目(CKJ2009005,CKJ2011004);福建省重点实验室开放课题基金(2008J1004-29)~~

摘　　要：背景:透骨消痛胶囊是福建中医药大学治疗骨关节炎的临床验方,以往的研究多集中于其对软骨的作用。目的:观察透骨消痛胶囊对成骨细胞增殖、分化及骨重塑相关因子表达的影响。方法:以透骨消痛胶囊干预大鼠成骨细胞 ROS17/2.8,分空白对照组和各浓度透骨消痛胶囊组,MTT 法测定细胞增殖,比色法检测成骨细胞分化的生物标志物碱性磷酸酶活性,ELISA 法检测骨钙素,茜素红染色观察骨矿化结节,实时定量 PCR 和 ELISA 法检测骨重塑相关因子骨保护素/核因子κB受体活化因子配体的表达。结果与结论:与对照组相比,质量浓度为 0.25-2 g/L 透骨消痛胶囊能显著促进 ROS17/2.8 细胞增殖(P < 0.05),上调碱性磷酸酶活性(P < 0.05)、骨钙素的表达水平(P < 0.05)和矿化结节面积(P < 0.05),增加骨保护素/核因子κB受体活化因子配体的比例(P < 0.05)。提示透骨消痛胶囊防治骨关节炎的作用机制可能与其促进成骨细胞的增殖、分化以及调节骨重塑平衡的作用有关。BACKGROUND：Tougu Xiaotong capsule is the clinical prescription for the treatment of osteoarthritis in Fujian University of Traditional Chinese Medicine, and the previous studies mainly focus on effect to cartilage. OBJECTIVE：To observe the effect of Tougu Xiaotong capsule on the proliferation and differentiation of osteoblasts as wel as the expressions of bone remodeling correlated factors. METHODS：Rat osteoblast-like cel line ROS17/2.8 cel s were incubated with Tougu Xiaotong capsule. The ROS17/2.8 cel s were divided into blank control group and Tougu Xiaotong capsule groups with different concentrations. The cel proliferation was determined by methylthiazolyldiphenyl-tetrazolium bromide assay. Osteoblast differentiation biomarkers alkaline phosphatase activity, osteocalcin and bone mineralized nodules were measured with colorimetry, enzyme-linked immunosorbent assay and alizarin red staining, respectively. The real-time PCR and enzyme-linked immunosorbent assay were used to detect the expressions of bone remodeling factors osteoprotegerin/receptor activator of nuclear factorκB ligand. RESULTS AND CONCLUSION：Compared with the control group, the Tougu Xiaotong capsule with the concentration of 0.25-2 g/L could significantly promote the ROS17/2.8 cel proliferation （P〈0.05）, up-regulate the alkaline phosphatase activity, osteocalcin expression level and mineralized nodules area, and increase the percentage of bone remodeling factors osteoprotegerin/receptor activator of nuclear factorκB ligand （P〈0.05）. The mechanism of Tougu Xiaotong capsule protecting osteoarthritis may partly result from the regulation of proliferation and differentiation of osteoblasts and bone remodeling.

关 键 词：组织构建 骨组织构建 透骨消痛胶囊 骨关节炎 软骨下骨 成骨细胞 增殖 骨保护素 核因子 ΚB 受体活化因子配体 骨重塑 省级基金 

分 类 号：R318[医药卫生—生物医学工程]