新型多孔偏磷酸钙复合膜的制备及细胞相容性  

Preparation and cytocompatibility of novel porous calcium metaphosphate composite membranes

作　　者：吴岳恒[1,2] 陈鹏[3] 麦丽萍[1,2] 张灵敏[3] 汤顺清[3] 余细勇[1,2] 

机构地区：[1]广东省医学科学院广东省心血管病研究所 [2]广东省人民医院医学研究中心,广东省广州市510100 [3]暨南大学生物医学工程研究所,广东省广州市510632

出　　处：《中国组织工程研究》2013年第34期6123-6130,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30901468)~~

摘　　要：背景:前期研究制备的多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜厚度较厚,且孔洞不均匀。目的:制备厚度薄且分布均匀的多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜,检测其细胞相容性及对细胞分化的作用。方法:通过相分离法制备厚度薄且分布均匀的无孔及多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜,检测其厚度及失重率。将人骨髓间充质干细胞分别与无孔及多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜共培养7d,扫描电镜观察复合膜的超微结构,流式细胞术分析复合膜上骨髓间充质干细胞的表面标记物。结果与结论:多孔和无孔复合膜的厚度分别为(0.041±0.005)mm和(0.058±0.004)mm,24h失重率分别为19.93%和7.64%。无孔复合膜上的偏磷酸钙粒子分布均匀,细胞完全铺展,呈梭形;多孔复合膜上的偏磷酸钙粒子分布均匀,孔径分布也均匀,孔径2-8μm,细胞全完铺展,呈多边形,有多个触角,部分细胞触角进入支架内部。无孔与多孔复合膜上的细胞均表达CD105、CD90、CD44、CD29及CD73,组间细胞阳性率差异无显著性意义。实验制备的聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜具有良好的细胞相容性,但无促细胞分化作用。BACKGROUND： Porous poly（3-hydroxybutyrate-co-4-hydroxybutyrate）/calcium metaphosphate composite membranes prepared previously is too thick and uneven in holes. OBJECTIVE： To prepare the thin even porous poly（3-hydroxybutyrate-co-4-hydroxybutyrate）/calcium metaphosphate composite membrane, and to evaluate the cytocompatibility and differentiation capacity. METHODS： Porous and nonporous, thin and even poly（3-hydroxybutyrate-co-4-hydroxybutyrate）/calcium metaphosphate composite membranes were prepared by phase separation method. Its thickness and weight loss rate were determined. Human bone marrow mesenchymal stem cells were cocultured with porous and nonporous poly（3-hydroxybutyrate-co-4-hydroxybutyrate）/calcium metaphosphate composite membranes for 7 days. Ultrastructure of composite membranes was observed under the scanning electron microscopy. Surface markers of the bone marrow mesenchymal stem cells on the composite membranes were analyzed using flow cytometry. RESULTS AND CONCLUSION： The thickness of the porous and nonporous composite membranes was （0.041 ± 0.005） mm and （0.058±0.004） mm. Weight loss rates of porous and nonporous composite membranes were respectively 19.93% and 7.64% at 24 hours. Calcium metaphosphate particles were evenly distributed in porous and nonporous composite membrane. Cells spread entirely, showing spindle shape. Calcium metaphosphateparticles were evenly distributed in porous composite membrane. Pore in porous composite membranes was also uniformly distributed, and pore size was about 2-8 μm. Cells spread entirely, showing polygonal shape with multiple tentacles. The tentacles of some cells entered into the scaffold. CD105, CD90, CD44, CD29 and CD73 expression was detected in porous and nonporous composite membranes. There was no significant difference in cell-positive rate. Poly（3-hydroxybutyrate-co-4-hydroxybutyrate）/calcium metaphosphate composite membranes prepared in this study has good biocompatibility and could not promote cell different

关 键 词：生物材料 材料生物相容性 膜生物材料 复合膜 聚3-羟基丁酸-4-羟基丁酸 偏磷酸钙 多孔 人骨髓基质干细胞 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]