几种常见牙周致病菌对羧甲基壳聚糖的降解效应  

Degradation of carboxymethyl chitosan by several common periodontal pathogens

作　　者：曹文帅[1] 邓婧[1] 徐全臣[1] 朱元祺[1] 

机构地区：[1]青岛大学医学院附属医院口腔内科,山东省青岛市266003

出　　处：《中国组织工程研究》2013年第34期6152-6158,共7页Chinese Journal of Tissue Engineering Research

基　　金：青岛市公共领域科技支撑计划项目(KZJ-26)~~

摘　　要：背景:近年来,羧甲基壳聚糖的体内降解已成为牙周组织工程和材料学领域中的研究热点,但在涉及羧甲基壳聚糖酶解的文献中,尚未找到关于牙周致病菌能够产酶降解羧甲基壳聚糖的报道。目的:研究几种常见牙周致病菌对羧甲基壳聚糖的降解作用。方法:制备质量分数1%和2%的羧甲基壳聚糖厌氧微生物培养基。采用透明圈法,分别挑取牙龈卟啉单胞菌、伴放线放线杆菌和黏性放线菌菌落划线接种于羧甲基壳聚糖厌氧微生物培养基中,置于厌氧培养袋中37℃恒温培养,观察结果,如果菌落周围出现明显的透明圈,即为阳性,说明细菌能够分解羧甲基壳聚糖,反之则为阴性,并测量菌落直径。结果与结论:牙龈卟啉单胞菌在1%羧甲基壳聚糖厌氧微生物培养基上生长良好,培养18d后菌落周围出现明显的透明圈;在2%羧甲基壳聚糖培养基上,牙龈卟啉单胞菌的生长受到抑制,未形成形态规则的菌落;伴放线放线杆菌和黏性放线菌在两种质量分数羧甲基壳聚糖厌氧微生物培养基中,菌落周围均未出现透明圈,培养2周后菌落干枯失去活性。提示牙龈卟啉单胞菌可降解较低质量分数的羧甲基壳聚糖,伴放线放线杆菌和黏性放线菌均不能降解羧甲基壳聚糖。BACKGROUND： In recent years, the degradation in vivo of carboxymethyl chitosan has become a research focus in the fields of periodontal tissue engineering and materials science. However, in the literature involving carboxymethyl chitosan enzymatic degradation, there is no report regarding whether common periodontal pathogens could degradate carboxymethyl chitosan. OBJECTIVE： To study the degradation of carboxymethyl chitosan by several common periodontal pathogens. METHODS： 1% and 2% carboxymethyl chitosan anaerobic microbiological culture media were prepared. The colonies of Porphyromonas gingivalis, Actinobacillus actinomycetemcomitan and Actinomyces viscosus were picked up streaking onto the carboxymethyl chitosan medium, respectively, which was then incubated in anaerobic culture bag at 37 ℃. If the see-through circle appeared around the colonies, the result was positive, indicating that the bacteria can degradate carboxymethyl chitosan; on the contrary, it was negative. Afterwards, the diameter of the colonies was measured. RESULTS AND CONCLUSION： It was observed that the colonies of Porphyromonas gingivalis grew well in the 1% carboxymethyl chitosan medium and the see-through circles appeared around the colonies after incubation for 18 days; meanwhile, the medium containing 2% carboxymethyl chitosan inhibited the growth of Porphyromonas gingivalis and the standard morphological colonies did not form well. The see-through circle didnot appear around the colonies of Actinobacillus actinomycetemcomitan and Actinomyces viscosus in the two concentrations of carboxymethyl chitosan media and the colonies were dried-up and lost activity after 2-week incubation. The results indicated that the lower concentration of carboxymethyl chitosan could induce Porphyromonas gingivalis to produce chitosanase and degradate carboxymethyl chitosan. Neither Actinobacillus actinomycetemcomitan nor Actinomyces viscosus could degradate carboxymethyl chitosan.

关 键 词：生物材料 生物材料与药物控释 膜生物材料 羧甲基壳聚糖 牙周致病菌 牙龈卟啉单胞菌 壳聚糖酶 透明圈 菌落 

分 类 号：R318[医药卫生—生物医学工程]