中国首次分离的二株基因Ⅰ型流行性乙型脑炎病毒全基因组序列分析（英文）  

Genome characterization of the first two genotype I strains

作　　者：冯云[1] 李灿委[2] 章域震[1] 杨卫红[1] 张海林[1] 

机构地区：[1]云南省地方病防治所/云南省病毒立克次体研究中心,大理671000 [2]大理学院公共卫生学院,大理671000

出　　处：《中国人兽共患病学报》2013年第9期829-835,共7页Chinese Journal of Zoonoses

基　　金：Supported by the National Natural Science Foundation of China(No.30560142);the China-U.S.Collaborative Program on Emerging&Re-Emerging Infectious Diseases(No.U19-GH000004)~~

摘　　要：目的分析中国首次分离的基因Ⅰ型流行性乙型脑炎病毒(JEV)全基因组的基因组特征。方法设计JEV全基因组扩增引物,RT-PCR扩增片断,PCR产物直接测序,拼接后获得全基因组序列。采用生物学软件进行同源性和系统进化分析。结果 1977和1982年分离自云南蚊虫的M28和BN82215病毒株基因组全长分别为10 969bp和10 970bp,5′非编码区均含有96个核苷酸,3′非编码区含有573和574个核苷酸。它们的开放阅读框(ORF)都从97到10 396位,共10 299个核苷酸,编码3 433个氨基酸。M28和BN82215株与来自GenBank的5个基因型JEV株的全基因组核苷酸和氨基酸同源性分别为78.4%~96.3%和91.4%~99.6%,与近几年国内外基因Ⅰ型流行株的同源性最高,进化关系最接近,都属于基因Ⅰ型。这两株病毒与JEV疫苗株SA14-14-2相比,有56个氨基酸差异,其中E基因有11个氨基酸差异,但都不属抗原关键位点。结论本研究阐明了中国首次分离的基因Ⅰ型JEV的全基因组分子特征,决定病毒抗原和毒力的E蛋白关键位点无明显变化。证实20世纪70和80年代云南省就有基因Ⅰ型JEV流行。Two strains of Japanese encephalitis virus （JEV）, M28 and BN82155, isolated from Yunnan Province respec tively in 1977 and 1982, are the first isolates of genotype I JEV in China. In this study, full-length genomes of them, one con taining an ssRNA molecule of 10 969 nt and the other 10 970 nt in length, were characterized. Both of them have a single 10 299-nucleotide open reading frames （ORF）, encoding 3 433 anaino acids. Compared with other representative strains of five genotypes retrieved from GenBank, M28 and BN82215 showed 78.4~^-96.3~ nucIeotide homology and 91.4^-99.6~ amino acid homology respectively, displaying close relationship with Genotype I JEV isolates circulating both in China and aboard in recent years. A total of 56 deduced amino acid residuals were observed differing from SA14-14-2 JE vaccine strain, with 11 amino acid mutations in the E gene. However, none of them was associated with known critical antigenic and virulent sites. In summary, characterization of first two genotype I JEV isolates from China demonstrated the prevalence of this genotype in Yunnan Province several decades ago.

关 键 词：流行性乙型脑炎病毒 基因Ⅰ型 全基因组 同源性分析 系统进化分析 

分 类 号：R512.321[医药卫生—内科学]