显示小球藻胞外多聚物的制样方法及CuO纳米颗粒在胞外多聚物中结构变化的电镜观察  

Sample preparation and observation of CuO ENPs structural changes in extracellular polymers of Chlorella using electron microscopy

作　　者：刘晓云[1] 张琛琛 王震宇 

机构地区：[1]中国海洋大学材料科学与工程研究院 [2]环境科学与工程学院,山东青岛266100

出　　处：《电子显微学报》2013年第4期352-355,共4页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(No.41073067);国家自然科学基金重大国际合作项目(No 41120134004)

摘　　要：建立一种保存单胞藻胞外多聚物结构的透射电镜快速制样方法,使单胞藻的胞外多聚物在透射电镜下清晰可见。在此基础上,观察单胞藻胞外多聚物对金属氧化物纳米颗粒的吸附作用、及其在胞外多聚物中发生的形态结构改变。探讨单胞藻胞外多聚物在改变金属氧化物纳米颗粒结构,最终导致纳米颗粒进入细胞这一过程中所发挥的可能作用。In this paper, a rapid transmission electron microscopy sample preparation methods saving extracellular polymer of unicellular algae which makes extracellular polymer visible clearly in TEM observation were established. On this basis, unicellular algae extracellular polymeric adsorption of metal oxide nanoparticles and metal oxide nanopartieles morphological changes occurring in the extracellular polymeric can be observed. In this way, we can investigate extraeellular polymeric＇ s possible role played in changing the structure of metal oxide nanoparticles and the process of leading to nanoparticles entering ceils eventually.

关 键 词：小球藻 胞外多聚物 纳米颗粒 

分 类 号：X13[环境科学与工程—环境科学]