经kinectin融合蛋白刺激的人B细胞诱导效应T细胞对肝癌细胞的杀伤  

Human B lymphocytes stimulated with kinectin protein induces effector T lymphocytes to kill hepatocellular carcinoma cells in vitro

作　　者：何少健[1] 韦力嘉[1] 宋琼[1] 肖瑞雪[1] 晁耐霞[1] 陈芳[1] 莫发荣[1] 农蔚霞[1] 

机构地区：[1]广西医科大学组织学与胚胎学教研室,广西壮族自治区南宁市530021

出　　处：《世界华人消化杂志》2013年第24期2418-2423,共6页World Chinese Journal of Digestology

基　　金：广西自然科学基金资助项目;No.桂科自0542068~~

摘　　要：目的:探讨经人动力素(kinectin)融合蛋白刺激的人外周血B细胞诱导效应T细胞杀伤肝癌细胞的效果.方法:取健康人外周血,分离出单个核细胞,加B细胞刺激因子及kinectin麦芽糖融合蛋白进行体外培养,尔后用尼龙毛柱分离T细胞,将其与肝癌细胞株7404混合培养.用乳酸脱氢酶法检测杀伤肝癌细胞的活性.结果:T细胞对肝癌细胞的杀伤率:空白对照组:3.40%±0.27%;实验组:38.48%±2.64%;阴性对照组:13.03%±2.38%.经统计软件分析,实验组杀伤率与其他组有明显差异.结论:经kinectin融合蛋白刺激的人外周血B细胞诱导的效应T细胞在体外对肝癌细胞株7404有杀伤的作用.AIM： To investigate whether human B lympho- cytes stimulated with kinectin protein induces effector T lymphocytes to kill hepatocellular car- cinoma cells. METHODS： Mononuclear cells were separated from healthy human peripheral blood and cul- tured in vitro. During cultivation, B cell activat-ing factor （BAFF） and MBP-kinectin were added. After being disassociated from cultured mono- nuclear cells using the method of nylon wool column, T lymphocytes were incubated together with hepatocellular carcinoma cell strain 7404. The killing effect of T lymphocytes on hepato- cellular carcinoma cells was detected by LDH release assay. RESULTS： The percentage of hepatocellular car- cinoma cells killed by T lymphocytes was as fol- lows： 3.40% ± 0.27% in the normal control group, 38.48% ± 2.64% in the experimental group, and 13.03% ± 2.38% in the negative control group. The percentage of hepatocellular carcinoma cells killed by T lymphocytes was significantly higher in the experimental group than in the normal control group and negative control group. CONCLUSION： Human B lymphocytes stimu- lated with kinectin fusion protein are able to induce effector T lymphocytes to kill hepatocel- lular carcinoma cells in vitro.

关 键 词：BAFF 人动力素 B细胞 效应T细胞 肝癌 细胞 

分 类 号：R735.7[医药卫生—肿瘤]