鼻咽癌中VEGF的表达与树突状细胞含量的相关分析及临床意义  

Correlation analysis of VEGF expression and the content of dendritic cells in nasopharyngeal carcinoma and its clinical significance

作　　者：苗北平[1,2] 张蕊石[1,3] 郭志红 孙焕吉[1,2] 孟庆国[1,2] 王晓彬[1,2] 

机构地区：[1]深圳市第二人民医院 [2]深圳大学第一附属医院耳鼻咽喉头颈外科,广东深圳518035 [3]深圳大学第一附属医院眼科,广东深圳518035 [4]深圳市罗湖中医院,广东深圳518000

出　　处：《山东大学耳鼻喉眼学报》2013年第4期62-65,69,共5页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：深圳市科技创新委重点资助课题(JCYJ20120613161724279)

摘　　要：目的探讨鼻咽癌组织中VEGF表达与树突状细胞含量的相关性。方法选取鼻咽癌组织和鼻咽部淋巴组织各25例,采用ELISA方法检测组织中VEGF的表达,采用流式细胞仪检测组织中树突状细胞和调节性T细胞的含量并分析其相关性。结果鼻咽癌组织中VEGF含量高于鼻咽部淋巴组织,组间差异具有统计学意义(t=12.324,P<0.01);鼻咽癌组织中CD4+淋巴细胞含量低于鼻咽部淋巴组织,组间差异具有统计学意义(t=14.304,P<0.01);而鼻咽癌组织中CD4+CD25+调节性T淋巴细胞含量高于鼻咽部淋巴组织,组间差异具有统计学意义(t=5.910,P<0.01);鼻咽癌组织中CD123+树突状细胞含量低于鼻咽部淋巴组织,组间差异具有统计学意义(t=7.866,P<0.01),鼻咽癌组织中CD11c+树突状细胞含量低于鼻咽部淋巴组织,组间差异具有统计学意义(t=3.411,P<0.01),鼻咽癌组织VEGF表达与CD4+CD25+淋巴细胞含量呈正相关(r=0.966,P<0.01),与CD123+树突状细胞含量呈负相关(r=-0.946,P<0.01),与CD11c+树突状细胞含量呈负相关(r=-0.954,P<0.01)。结论鼻咽癌组织中VEGF表达与鼻咽癌组织中CD4+、CD25+调节性T细胞和树突状细胞的含量有关,可能参与鼻咽癌的免疫逃逸。Objective To investigate the correlation between VEGF expression and the content of dendritic cells in nasopharyngeal carcinoma. Methods 25 cases of nasopharyngeal carcinoma and 25 cases of nasopharyngeal lymphoid tissue were collected. The expressions of VEGF in the nasopharyngeal carcinoma and nasopharyngeal lymphoid tissues were detected by ELISA. The content of dendritic cells and regulatory T cells in the nasopharyngeal carcinoma and nasopharyngeal lymphoid tissues were detected by flow cytometry. Then the correlation between VEGF expression and the content of dendritic cells was analyzed. Results： Expression of VEGF in nasopharyngeal carcinoma was higher than that in nasopharyngeal lymphoid tissues （ t = 12. 324, P 〈 0.01 ） ; the content of CD4 ＋ lymphocyte in nasopharyngeal carcinoma was lower than that in nasopharyngeal lymphoid tissues （ t = 14. 304, P 〈 0.01 ）. While the content of CD4 ＋ CD25 ＋ regulatory T cells in nasopharyngeal carcinoma was higher than that in nasopharyngeal lymphoid tissues, and the difference was statistically significant（ t = 5. 910, P 〈 0.01 ）. The content of CD123 ＋ dendritic cells in nasopha- ryngeal carcinoma was lower than that in nasopharyngeal lymphoid tissues, and the difference was statistically signifi- cant（ t = 7. 866, P 〈 0.01 ）. The content of CD11c ＋ dendritic cells in nasopharyngeal carcinoma was lower than that in nasopharyngeal lymphoid tissues, and the difference was statistically significant（ t = 3.411, P 〈 0.01 ）. The expression of VEGF was positively correlated with the content of CD4 ＋ CD25 ＋ lymphocytes in nasopharyngeal carcinoma （ r = 0. 966, P 〈 0.01 ）, and negatively correlated with the content of CD123 ＋ dendritic cells （ r = - 0. 946, P 〈 0.01 ） andnegatively correlated with the content of CD11c ＋ dendritic ceils （ r = - 0. 954, P 〈 0.01 ）. Conclusion Expression of VEGF is related to the content of CD4 ＋ CD25 ＋ regulatory T cells and the content of dendritic ceils in nasopharynge

关 键 词：鼻咽癌 VEGF 树突状细胞 调节性T细胞 

分 类 号：R739.62[医药卫生—肿瘤]