重金属铅离子(Pb^(2＋))对两株螺旋藻生长影响的研究  

Pb^(2+) impacts on growth of two Spirulina strains

作　　者：李勇勇[1] 赵楠[1] 李善策[2] 邵明飞[3] 郁章玉[1,4] 秦松[5] 

机构地区：[1]曲阜师范大学化学与化工学院,曲阜273165 [2]中南大学资源加工与生物工程学院,长沙410083 [3]南京农业大学资源与环境科学学院江苏省海洋生物学重点实验室,南京210095 [4]菏泽学院化学与化工系,菏泽274015 [5]中国科学院烟台海岸带研究所生物资源实验室,烟台264003

出　　处：《生物学杂志》2013年第4期37-41,共5页Journal of Biology

基　　金：海洋公益性行业科研专项经费资助(200905021-3)、(201205027);山东省自然科学基金项目(ZR2012DQ015);广东省中国科学院全面战略合作项目(2011A090100040)

摘　　要：以来自两个不同企业产业化的螺旋藻作为出发藻株A、藻株B,从生物量、藻胆蛋白、可溶性蛋白、半数致死浓度4个方面研究藻株对重金属铅离子(Pb2+)的耐受性,为解析耐受机理积累研究基础。实验结果表明,两株藻对Pb2+的耐受性存在明显差异,藻株B比藻株A对铅的耐受性强。藻株A:Pb2+浓度低于1 mg/L,促进其生长;Pb2+浓度为10～40 mg/L,对其无明显影响;Pb2+浓度大于50 mg/L时,抑制其生长;Pb2+浓度为100 mg/L,藻丝体降解死亡;96 h半数致死浓度(EC50)为61.66 mg/L。藻株B:Pb2+浓度低于20 mg/L,对其无明显影响;Pb2+浓度为20 mg/L,促进其生长;Pb2+浓度大于50 mg/L,抑制其生长;Pb2+浓度为100 mg/L,藻丝体降解死亡;96 h半数致死浓度(EC50)为72.44 mg/L。当Pb2+浓度为20 mg/L时,藻蓝蛋白(PC)和水溶性蛋白均具有较高的积累量,与其正常生长情况相似。To investigate the impacts of heavy metal ions（Pb2＋） on spirulina biomass,phycobiliprotein,soluble proteins,and the median lethal concentration,a comparative study between two industrialized spirulina strains（A and B） was conducted.Experimental data indicated that the response of strain A was quite different from strain B with different Pb2＋,and that strain B was more tolerant to Pb2＋ than strain A.With Pb2＋ concentration lower than 1 mg/L,growth of strain A was promoted;with Pb2＋ concentration from 10 to 40 mg/L,no obvious effect was found;with Pb2＋ concentration more than 50 mg/L,inhibited;with Pb2＋ concentration 100 mg/L,died;the 96 h-EC50 of strain A was 61.66 mg/L.With Pb2＋ concentration lower than 20 mg/L,no obvious effect was found on strain B;with Pb2＋ concentration 20 mg/L,strain B growth was promoted;with Pb2＋ concentration more than 50 mg/L,inhibited;with Pb2＋ concentration 100 mg/L,died;the 96 h-EC50 of strain A was 72.44 mg/L.When Pb2＋ concentration was 20 mg/L,content of phycocyanin and water-soluble proteins were higher,consistent with the normal growth.

关 键 词：螺旋藻 铅离子 生物量 96h-EC50 

分 类 号：X131.2[环境科学与工程—环境科学]