冻融抗原冲击致敏的树突状细胞对结肠癌小鼠的治疗作用  

Treatment of Established Colon Carcinoma-Bearing Mice by Vaccination with Dendritic Cells Pulsed with Tumor Cell Lysates

作　　者：唐华[1] 银平章[1] 孔令非[1] 曹雪涛[2] 

机构地区：[1]河南省人民医院生物治疗中心,郑州450003 [2]第二军医大学免疫学教研室,上海200433

出　　处：《中国肿瘤生物治疗杂志》2000年第3期199-202,共4页Chinese Journal of Cancer Biotherapy

基　　金：上海市优秀学科带头人资助计划!(97XD14 0 6 );高等学校优秀青年教师教学科研奖励计划!(TRAPOTY)

摘　　要：目的 :研究冻融的肿瘤抗原冲击致敏的骨髓来源的树突状细胞 (DC)对结肠癌小鼠是否具有治疗作用。方法 :用小鼠结肠癌细胞株C2 6冻融抗原体外冲击致敏BALB/c小鼠骨髓来源的DC ,观察其体外刺激荷瘤小鼠脾细胞增殖的能力及其诱导的CTL对C2 6肿瘤细胞的杀伤活性 ;体内以 3× 10 5DC /只一次或多次接种于已荷瘤 10d结肠癌的小鼠同侧腹股沟皮下 ,观察抗原冲击的DC对肿瘤生长的抑制作用以及对荷瘤小鼠生存期的影响。结果 :体外抗原冲击致敏的DC能显著刺激荷瘤小鼠脾脏T细胞增殖 ,其诱导的CTL对C2 6肿瘤细胞具有显著的杀伤作用 ,在效靶比为 10∶1,5∶1,2 .5∶1时其杀伤率分别 88 1% ,71.4%和 5 0 .0 % ;抗原冲击致敏的DC体内多次皮下免疫后对肿瘤的生长具有显著的抑制作用 ,能显著延长荷瘤小鼠的生存期。一次性DC体内免疫接种对荷瘤小鼠的治疗作用不明显。结论Objective: To investigate whether vaccination with dendritic cells pulsed with C26 colon carcinoma cell lysates has the therapeutic effect on the established C26 colon carcinoma bearing mice. Methods: Dendritic cells generated from murine bone marrow were pulsed with C26 tumor lysates. The T cell stimulating activity and CTL induction were detected. The dendritic cells were injected s.c (3 ×10 5/muose) only once on the day 10 or 4 times on the day 10, 15, 20, 29 repeatedly after the C26 tumor cells were inoculated. The therapeutic effect of DC was observed. Results: DC could stimulate proliferation of syngeneic T cells potently, and induce CTL activity markedly. The tumor growth was significantly suppressed and the survival time of tumor bearing mice was prolonged when the DC pulsed with tumor lysates were injected s.c repeatedly. Conclusion: Vaccination with DC pulsed with tumor lysates has the potent therapeatic effect on the established colon carcinoma bearing mice.

关 键 词：树突状细胞 免疫治疗 结肠癌 冻融抗原 

分 类 号：R735.350.5[医药卫生—肿瘤] R730.51[医药卫生—临床医学]