蓝莓SRAP-PCR反应体系的建立和优化及其遗传多样性分析  

Optimization and Establishment of SRAP-PCR Reaction System of Vaccinium ssp. and Analysis of the Genetic Diversity

作　　者：傅冰[1] 洪震[1] 刘跃钧 潘芝梅[1] 

机构地区：[1]丽水职业技术学院环境工程分院,浙江丽水323000 [2]丽水市林业科学研究院,浙江丽水323000

出　　处：《北方园艺》2013年第17期95-99,共5页Northern Horticulture

摘　　要：以9个品种蓝莓为试材，采用L16（4^5）正交实验设计建立和优化了蓝莓SRAP-PCR反应体系，并利用该体系分析了供试蓝莓品种之间的遗传多样性。结果表明：蓝莓SRAP—PCR反应的最佳体系（20μL）为：MgCl22．5mmol／L、dNTPs100μmol／L、TaqDNA聚合酶0．8U、引物0．2t~mol／L、DNA40ng、1×ReactionBuffer；9个品种蓝莓的遗传相似性系数分布于0．1818-1．0000之间，平均遗传相似性系数为0．6359，存在较高的遗传多样性。基于UPGMA的聚类分析将供试材料分成4个类群，主坐标分析同样将供试材料分成4个类群，二者结果一致。Taking 9 varieties of blueberry as material,the SRAP-PCR reaction system of Vaccinium ssp. was established and optimized by L16 （4^5 ） orthogonal experimental design. The genetic diversity of them was analyzed by the optimal reaction system. The results showed that the optimal and stable SRAP-PCR system was as follows：2. 5 mmol,/I. Mg^2＋ , 100μmol/L dNTPs,0. 8 U Taq polymerase,0. 2 μmol/L primers and 40 ng DNA template, 1 × Reaction Buffer,within total 20μL reaction solution. The GS coefficients among the 9 varieties of blueberry were ranged from 0. 1818 to 1. 0000 with an average of 0. 6359, this suggested that the genetic variation was high. These varieties were divided into four groups oil the UPGMA dendrogram constructed from GS coefficients,and also divided into four groups by the method of principal coordinates analysis. The UPGMA and C-S coefficients results was the same.

关 键 词：蓝莓 SRAP-PCR 优化 遗传多样性 

分 类 号：S663.9[农业科学—果树学]