北冬虫夏草寄主体培养关键技术研究  

Study on the Key Cultivation Technology of North Cordyceps Host

作　　者：秦秀丽[1] 邢力[1] 尹锐[1] 

机构地区：[1]吉林农业科技学院,吉林吉林132101

出　　处：《北方园艺》2013年第17期157-160,共4页Northern Horticulture

基　　金：吉林省教育厅"十一五"科学技术研究资助项目(吉教科合字[2011]第274号);吉林省科技厅发展计划资助项目(201105071)

摘　　要：以野生北冬虫夏草为试材，研究了北冬虫夏草寄主体培养关键技术。结果表明：液体菌种的最佳菌龄为7～9d，用臭氧水进行蛹体表面消毒其效果最佳，接种时在蚕蛹翅的下缘进行接种易操作，出血少，僵化率高；通过正交实验确定发菌时适宜的条件为温度20-22℃，空气湿度60％，在无光条件下进行发菌，其僵化率最高；出草的适宜条件为温度20～22℃，空气湿度60％～70％，光照强度250-500Ix，其蛹虫草长势最好，得到的蛹虫草干重高。Taking wild North Cordyceps as material, the key cultivation technology of North Cordyceps host was conducted. The results showed that the best bacteria age of liquid strains was 7-9 d,ozone solution was the best surface disinfection of pupa body,it was easy operation by inoculate on the lower edge of Silkworm pupa wing and bleeding less, with high rigid rate. Suitable conditions were determined by the orthogonal test, the results showed that the suitable temperature for the hyphae growth was 20~22~C ,relative air humidity was 60% ,the pupa rigid rate was the highest in no light condition. The suitable conditions for the sporocarp growth as follows, temperature for 20-22℃, relative air humidity for 60% -70%,light density for 250-500 ix, under this condition, the North Cordyceps growth was the best, the dry weight of North Cordyceps was the highest.

关 键 词：北冬虫夏草 液体菌种 发菌 僵化率 出草 

分 类 号：S567[农业科学—中草药栽培]