厌氧氨氧化菌混培物保藏方法的研究  

Preservation of ANAMMOX bacteria

作　　者：汪彩华[1,2] 郑平[1] 蔡靖[3] 陈婷婷[1] 

机构地区：[1]浙江大学环境工程系,浙江杭州310029 [2]北京天地人环保科技有限公司,北京100176 [3]浙江工商大学环境工程系,浙江杭州310018

出　　处：《中国环境科学》2013年第8期1474-1482,共9页China Environmental Science

基　　金：国家自然科学基金资助项目(31070110);国家"863"项目(2009ZZ06311)

摘　　要：厌氧条件下,采用了4℃以(NH4)2SO4为保藏基质、自然环境温度以(NH4)2SO4为保藏基质、4℃用NH4Cl代替(NH4)2SO4作为保藏基质3种方法保藏厌氧氨氧化菌混培物.结果表明,4℃以(NH4)2SO4为保藏基质的方法保藏厌氧氨氧化菌混培物最有效,但自然环境温度以(NH4)2SO4为保藏基质的方法保藏厌氧氨氧化菌混培物最经济;在保藏试验的5个月内,3种保藏方法下的厌氧氨氧化活性均先快速下降后趋于稳定,趋于稳定时的活性保留率分别为75.3%,66.3%和47.3%;恶劣的生存环境下,胞外多聚物不仅可维持厌氧氨氧化菌混培物颗粒结构的稳定性,还可使菌群聚集成团从而增大菌群密度以减少代谢过程中的能量损失;无外界颜色干扰时,厌氧氨氧化菌混培物颜色由血红素c含量决定,且与活性直接相关.Under anaerobic conditions, three methods on preservation of ANAMMOX bacteria were studied, which were at 4℃ with （NH4）2SO4 as storage matrix,at natural temperature with （NH4）2SO4 and at 4℃ with NH4C1, respectively. At 4℃ with （NH4）2SO4was the most effective method, but at ambient temperature with （NH4）2SO4was the most economical method. During the 5-month preservations, ANAMMOX activities with the three methods decayed rapidly at the beginning, and stabilized gradually later on. When activities gone steady, the survival activities with the three methods were 75.3%, 66.3% and 47.3%, respectively. Extracellular polymers not only maintained the structural integrity of ANAMMOX granules, but also increased the cell density for decreasing the energy loss and the injury of starvation in severe environment. Without color interference, the color of ANAMMOX bacteria was directly correlated with the content ofheme c, which could reflect the activities of ANAMMOX bacteria.

关 键 词：厌氧氨氧化菌混培物 温度 基质种类 保藏性能 

分 类 号：X703.5[环境科学与工程—环境工程]