HPLC同时测定珠子草中没食子酸、短叶苏木酚和鞣花酸的含量  

Simultaneous Determination of Galic Acid, Brervifolincaboxylic Acid and Ellagic Acid in Phyllanthus Niruri Linn by HPLC

作　　者：梁云 李彩东[2] 王信[2] 

机构地区：[1]兰州市肺科医院,兰州730046 [2]兰州市第二人民医院,兰州730046

出　　处：《中国现代应用药学》2013年第8期880-882,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：兰州市科技局立项项目(2007-1-75)

摘　　要：目的建立同时测定珠子草中没食子酸、短叶苏木酚和鞣花酸的HPLC法，并对珠子草该3种成分进行含量测定。方法色谱柱：ShimazuC18（150mm×4．6mm，5μm）；流动相：0．1％磷酸（A）．乙腈（B）梯度洗脱，流速为0．8mL·min^-1；柱温：室温；检测波长：270nm；进样量10此。结果没食子酸、短叶苏木酚和鞣花酸含量分别在0．6~9．6μg、0．525~8．4μg、0．475～7．6μg内与峰面积呈良好的线性关系。没食子酸的平均含量为0．1962％，短叶苏木酚为o．5182％，鞣花酸为0．4110％。结论本方法操作简便，准确度高，可用于珠子草中没食子酸、短叶苏木酚和鞣花酸3种成分含量的测定。OBJECTIVE To establish the method for determination of galic acid, brervifolincaboxylic acid and ellagic acid in Phyllanthus niruri Linn. METHODS HPLC separation was carried on Shimazu C18（150 mm×4.6 mm, 5 μm） column with mobile phase consisted of acetonitrile and 0.1% phosphoric acid in a program of gradient elution. Flow rate： 0.8 mL.min-1, column temperature： room temperature, and the detection wavelength was 270 nm. RESULTS The linear range of galic acid, brervifolincaboxylic acid and ellagic acid were 0.6-9.6 μg, 0.525-8.4 μg, 0.475-7.6 μg, respectively; and the content of them in Phyllanthus niruri Linn were 0.196 2%, 0.518 2%, 0.411 0%, respectively. CONCLUSION The developed method is simple, accurate and feasible. It＇s suitable for the determination of galic acid, brervifolincaboxylic acid and ellagic acid in Phyllanthus niruri Linn.

关 键 词：珠子草 没食子酸 鞣花酸 短叶苏木酚 同时测定 

分 类 号：R917.101[医药卫生—药物分析学]