应用16S rRNA基因序列分析与细菌生化反应鉴定湖生代夫特菌  

Identification of Delftia lacustris by 16S rRNA gene sequencing and biochemical reactions

作　　者：陈茶[1] 屈平华[1] 黄彬[2] 刘建平[1] 张妮[3] 袁慧[3] 

机构地区：[1]广东省中医院检验医学部,广东广州510006 [2]中山大学附属第一医院检验医学部,广东广州510080 [3]广州中医药大学第二临床医学院,广东广州510006

出　　处：《中华医院感染学杂志》2013年第15期3572-3574,共3页Chinese Journal of Nosocomiology

摘　　要：目的对某医院术后感染中分离到的4株不常见病原菌进行菌种鉴定。方法采用16SrRNA基因序列的测定、细菌形态学以及商品化的VITEK-2GN、API 20NE系统相结合的多相分类学方法。结果该4株菌均为需氧的革兰阴性非发酵菌,其氧化酶阳性,在血平板、巧克力平板、麦康凯平板等常用培养基上生长良好;采用VITEK-2GN与API 20NE系统,该4株菌均鉴定为食酸代夫特菌/食酸丛毛单胞菌,并显示为极好的鉴定度;但16SrRNA基因序列分析的结果显示,该4株菌与食酸代夫特菌的相似度仅为98.4%,而与鹤原代夫特菌、湖生代夫特菌的同源性更高,分别为99.9%和100.0%;其甘露醇同化试验与苹果酸同化试验阳性,与湖生代夫特菌一致而不同于鹤原代夫特菌。结论该术后感染的4株病原菌均为湖生代夫特菌,16SrRNA基因序列分析能提供有力的遗传学证据。OBJECTIVE To identify 4 infrequently isolated bacteria isolated from the patients with postoperative infections. METHODS The bacterial 16S rRNA gene sequence-based identification, commerical VITEK-2 automate systems, API 20NE strips and VITEK-2 GN kits were performed to all of the 4 isolates respectively. RESULTS All 4 strains were glucose non-fermenting gram-negative bacilli with positive oxidase reaction, which were well- growing on blood agar, haemophilus chocolate agar and Maconkey agar. They would be mistakenly identified to Delftia acidovorans with a excellent confidence by API 20NE and VITEK-2 GN kits, their 16S rRNA gene sequences were only 98.4 % identity to the valid published D. acidovorans, but more closely related to D. tsuru- hatensis and D. lacustri with 99.9% and 100.0% identity. And the positive reaction of carbon utilization of mani- tol and malate were identified to D. lacustri and differentiated to D. tsuruhatensis. CONCLUSION The pathogens of the postoperative infection are identified as D. lacustri, and the comparative analysis based on 16S rRNA gene sequences is a useful method to identify such problematic and newly nomenclated bacteria.

关 键 词：代夫特菌属 RNA 核糖体 16S RRNA 基因测序鉴定 

分 类 号：R378[医药卫生—病原生物学]