MSTN RNAi双元表达载体构建及效果试验  

Construction of MSTN RNAi binary Vector

作　　者：胡兰[1] 隋世慧[1] 吴丹[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,辽宁沈阳110866

出　　处：《中国兽医杂志》2013年第7期11-13,17,共4页Chinese Journal of Veterinary Medicine

摘　　要：为了建立有效抑制MSTN基因表达的方法,本试验利用基因克隆技术构建MSTN RNAi双元表达载体,并利用脂质体介导转染成肌细胞、用SDS-PAGE和Western blottingf法检测蛋白质的表达。试验结果表明,以TA载体为克隆载体,pHANNIBAL为中间载体,可成功构建pEGFP-N1-M1-M2双元表达载体,将其转染成肌细胞48h后,该双元表达载体可明显抑制成肌细胞MSTN基因的表达。To inhibit the expression of MSTN, MSTN RNAi binary expression vector was constructed with gene cloning technology, which was transfected into myoblast cells by lipidosome, and the expression protein was detected by SDS-PAGE and Western blot. The result shows that pEGFP-N1-MSTN can be constructed successfully by using TA vector as cloning Vector and pHANNIBAL as the middle carrier vector. The binary expression vector could inhibit MSTN gene expression in myoblast cell 48h after trans- fection.

关 键 词：MSTN基因 RNAI 双元表达载体 成肌细胞 

分 类 号：D852.2[政治法律—政治学]