高度集约化奶牛场奶牛乳房炎病原菌的分离计数及感染规律  

Isolation and Counting of mastitis Pathogen and its Rules of Infection on highly Intensive Dairy farms

作　　者：甘宗辉[1] 杨章平[1] 刘贤慧[1] 张美荣[1] 李云龙[1] 毛永江[1] 陈飞 陈亮 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]上海牛奶集团,江苏大丰224100

出　　处：《中国兽医杂志》2013年第7期24-26,29,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金(3127407);国家863项目(2008AA10Z144);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：该试验旨在研究病原菌感染与奶牛乳房炎发病之间的关联性。根据细菌分离鉴定程序和菌落总数计数法测定了636份乳样,涉及无乳房炎乳、不同等级的隐型乳房炎乳、临床型乳。结果表明,该牧场奶牛所患的乳房炎是以单种细菌感染为主,并且在一定的细菌感染比例下,乳房炎的发病程度与杆菌的相对比例正相关,与葡萄球菌的相对比例负相关;同时,乳房炎的发病程度还与菌落总数的含量正相关。无乳房炎乳、隐型乳房炎乳、临床型乳房炎乳中的菌落总数差异极显著(P<0.01)。结果提示,本场乳房炎的发病程度与杆菌所占的比例和菌落总数成正相关关系。The article is intended to study the links between pathogenic bacteria infection and mastitis. Based on the isolation and identification of bacteria and colony count method 636 milk samples were ana- lyzed, including the normal breast, invisible, clinical mastitis mastitis milk. The result shows that： The ranch mastitis was dominated by a single bacterial infection,and with the rising in the proportion of Lacto- bacillus, the reducing the proportion of Staphylococcus as well as an increase in the aerobic bacterial count,mastitis levels gradually increased. The colonies in normal milk, invisible and clinical mastitis milk had a significant difference compared with the positive（P〈0.01）. Results suggest that： with the increase of the proportion of Mycobacterium and the total number of bacterial colony the incidence of mastitis also increase therets a positive correlation between them.

关 键 词：细菌 乳房炎 菌落总数 

分 类 号：S852.61[农业科学—基础兽医学] S857.26[农业科学—兽医学]