受体上皮细胞灌注后大鼠气管异位移植再上皮化过程的研究  

Study on the process of reepithelization after rat heterotopic tracheal graft with recipient epithelial cells perfusion

作　　者：张轶[1] 胡学飞[1] 陈昶[1] 

机构地区：[1]同济大学附属上海市肺科医院,上海200433

出　　处：《现代中西医结合杂志》2013年第25期2752-2756,共5页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：上海市科委资助项目(114119a9400)

摘　　要：目的研究受体上皮细胞灌注后大鼠气管异位移植再上皮化的具体的演进过程。方法建立大鼠气管异位移植模型后,将实验动物完全随机分为6组,在移植后第3天、第7天、第14天、第21天、第28天、第35天,分别随机处死一组大鼠(n=10),取出移植气管,分切后进行以下检测:组织形态学评价、透射电镜观察、上皮细胞分化鉴定(CK14、CK18和CFTR)、上皮细胞增殖能力检测(Ki67)和上皮细胞纤毛比例测定。结果在移植后的第3天,气管内壁局部覆盖有无纤毛的扁平上皮细胞;第7天,气管内壁完整覆盖有无纤毛的扁平上皮细胞;第14天,上皮细胞层为扁平细胞和柱状细胞交替;第21天,气管内壁覆盖完整的柱状上皮细胞,偶见刷状缘;第28天,气管内壁覆盖完整的柱状上皮细胞,可见较明显的刷状缘,透射电镜发现假复层结构明显;第35天的表现与第28天相仿。结论受体上皮细胞灌注后大鼠气管异位移植再上皮化的演进过程为:灌注后细胞迅速附壁,其中成功附壁的上皮细胞开始增殖,进而完整覆盖气管内壁,形成单层扁平上皮组织,而后上皮细胞开始分化,最终成为成熟的假复层纤毛柱状上皮组织。Objective It is to study the process of reepithelization after rat heterotopic tracheal graft with recipient epithelial cells perfusion. Methods After establishment of heterotopic subcutaneous tracheal transplantation model in rat, the animals were randomly divided into six groups. Rats （n = 10） were killed on 3, 7, 14, 21, 28 and 35 days respectively after trans- plantation and then the implanted tracheae were harvested. In all specimens, histological and ultrastructural changes were e- valuated, differentiation （CK14, CK18, CFTR） and proliferation （Ki67） of epithelium were identified. Results On 3 days after transplantation, the regional areas of tracheal inner surface were covered by non-eiliated flare cells; On 7 days, the whole areas of tracheal inner surface were covered by non-ciIiated flate cells ; On 14 days, the whole areas of tracheal inner surface were covered by the mixture of flate ceils and columnar cells; On 21 days, the whole areas of tracheal inner surface were cov- ered by columnar cells ; On 28 days, the whole areas of tracheal inner surface were covered by eiliated columnar cells arranged in pseudostratified structure; The results on 35 days after transplantation were similar to those on 28 days. Conclusion Cells adhered to tracheal inner wall quickly after perfusion of cells suspension. Then the adherent flate cells started to proliferate un- til the whole inner surface was covered. And the cells started to differentiate. Finally, mature pseudostratified ciliated colum- nar cells appeared.

关 键 词：气管移植 裸气管 再上皮化 免疫抑制治疗 

分 类 号：R73-351[医药卫生—肿瘤]