L型钙离子通道在体外鼠牙胚发育中的表达  

Expression of L-type calcium channel in mouse molar development in vitro

作　　者：李艳[1] 赵守亮[1] 梁丽[1] 许多[1] 

机构地区：[1]同济大学附属口腔医院牙体牙髓科,上海200072

出　　处：《同济大学学报（医学版）》2013年第3期20-23,共4页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金(30471889);上海市科委医学引导项目(10411964600)

摘　　要：目的观察L型钙离子通道在体外培养的牙胚发育过程中表达的时空特异性。方法取20只18 d胎龄的ICR孕鼠下颌第一磨牙牙胚,用Trowell培养系统进行培养。分别培养4 d、7 d,用小鼠抗Cav1.1单克隆抗体、兔抗Cav 1.2、Cav 1.3、Cav 1.4多克隆抗体进行免疫组织化学染色,光镜下观察在小鼠牙胚发育的不同阶段L型钙离子通道各种亚型的表达情况。结果 Cav 1.2和Cav 1.3在E 18 d、培养4 d、培养7 d时在不同的部位都有表达,但表达的强弱不同。未见有Cav 1.1和Cav 1.4的表达。结论 L型钙离子通道在牙胚发育过程中的表达具有时空特异性。Objective To investigate the expression of L-type calcium channel in mouse molar development in vitro.Methods Twenty lower first molars were taken from mandibles of 18-day fetal mice and cultured for 4 or 7 days in Trowel cultured systems.The expression of L-type calcium channel in fetal mouse molars development were immunohistocheminally stained with mouse monoclonal antibody Cav 1.1 and rabbit polyclonal antibody Cav 1.2,Cav 1.3,Cav 1.4.Results The Cav 1.2 and Cav 1.3 were expressed in different period and different parts of tooth germ development,but the expression of Cav 1.1 and Cav 1.4 was not detected.Conclusion The expression of L-type calcium channel shows spatial and temporal characteristics during the development of fetal mouse molars in vitro.

关 键 词：牙胚 Trowell器官培养系统 L型钙离子通道 

分 类 号：R781.1[医药卫生—口腔医学]