儿童白血病患者血浆12种微量元素含量变化研究  

Study on plasma contents of 12 trace elements in children with leukemia

作　　者：钟静霞[1] 舒晓亮[2] 徐涵[2] 康凯[2] 柳先丽[2] 蔡东联[3] 

机构地区：[1]同济大学附属同济医院儿科,上海200065 [2]同济大学附属东方医院营养科,上海200120 [3]第二军医大学附属长海医院营养科,上海200433

出　　处：《同济大学学报（医学版）》2013年第4期86-89,共4页Journal of Tongji University(Medical Science)

基　　金：上海市科技发展基金(074119048)

摘　　要：目的采用电感耦合等离子体质谱法(ICP-MS)测定儿童白血病血浆12种微量元素含量变化。方法300例白血病儿童和60例健康儿童的血浆样品,样品经硝酸(HNO_3)-过氧化氢(H_2O_2)湿法消解,用Triton X-100和正丁醇稀释,检测血浆微量元素浓度。结果该分析方法12种微量元素在0~100μg/L范围内线性相关良好,r>0.999;检出限为0.000 2~1.12μg/L;平行测定的RSD为0.87%~4.0%;加标回收率为91.0%~110%。结论白血病儿童血浆Zn、Fe的浓度低于健康儿童,而血浆AS、Pb、Cu浓度高于健康儿童。Objective To determine the plasma contents of 12 trace elements in children with leukemia by inductively coupled plasma mass spectrometry （ICP-MS）. Methods Plasma samples were collected from 300 children with leukemia and 60 health children. The samples were diluted with Triton X-100 and butanol after digested with HNO3-H202, and the contents of 12 trace elements were measured by ICP^MS method. Results In meaurement of 12 plasma trace elements, the limits of detection ranged from 0. 000 2 μg/L to 1.12 μg/L with the precision （RSD） of 0.87% - 4.0%. The spike recovery rates for most elements were 91.0% - 110%. Conclusion Results show that the plasma levels of Zn and Fe are lower, and As, Pb and Cu are higher in leukemia children than those in healthy children.

关 键 词：电感耦合等离子体质谱 儿童白血病 微量元素 

分 类 号：R733.7[医药卫生—肿瘤]