涝渍胁迫下白桦根系可溶性糖和淀粉含量与电阻抗的相关性分析  

The Correlation Analysis of Soluble Sugar and Starch Contents with Electrical Impedance in Betula platyphylla Suk. Roots under Waterlogging and Flooding Stresses

作　　者：孟昱[1] 邸葆[2] 张钢[2] 封新国 徐成立[4] 田军[4] 

机构地区：[1]河北农业大学林学院,河北保定0710011 [2]河北农业大学园艺学院,河北保定071001 [3]河北省林业厅,石家庄050000 [4]河北省木兰围场国有林场管理局,河北承德068450

出　　处：《生物物理学报》2013年第6期450-460,共11页Acta Biophysica Sinica

基　　金：国家林业局948项目(2011-4-08)~~

摘　　要：以白桦(Betula platyphylla Suk.)2年生实生苗为材料,测定根系在涝渍胁迫下可溶性糖和淀粉含量与电阻抗图谱(electrical impedance spectroscopy,EIS)参数的变化,分析可溶性糖和淀粉含量与EIS参数的相关性。试验分对照、渍水和淹水3个处理组,处理时间为60 d。结果表明:1)随着涝渍胁迫处理时间的延长,对照组可溶性糖含量呈整体缓慢上升趋势;渍水组呈先下降,后上升的趋势;淹水组呈先上升,后下降的趋势。2)随着涝渍胁迫处理时间的延长,对照和渍水组淀粉含量都呈整体上升的趋势;淹水组呈整体下降趋势。3)电阻抗图谱参数在涝渍胁迫处理期间均发生变化,且胞外电阻率(re)和弛豫时间(τ)的变化趋势相同。4)相关性分析结合通径分析表明,在涝渍胁迫期间,可溶性糖含量对re的影响最大,二者呈显著的正相关;淀粉含量对弛豫时间分布系数(ψ)的影响最大,二者呈显著的负相关。说明电阻抗图谱参数能够有效地表征涝渍胁迫下白桦根系可溶性糖和淀粉的变化,电阻抗技术是研究林木根系在逆境胁迫下生理状况的一种有效方法。Two-year-old seedlings of white birch （Betula platyphylla Suk.） were used to measure soluble sugar and starch contents as well as electrical impedance spectroscopy （EIS） parameters of roots under waterlogging and flooding stresses. The correlations of the EIS parameters with tested soluble sugar and starch contents were analyzed. Three treatments, the control, waterlogging and flooding, were carried out with duration of 60 days. The results showed that： 1） Following the prolong of stress treatments time, soluble sugar content of the control decreased slowly; that of the waterlogging treatment decreased firstly, and increased later on; that of the flooding treatment increased firstly, and then decreased. 2） Following the prolong of stress treatments time, starch content of the control and waterlogging treatments increased, and that of flooding treatment decreased. 3） EIS parameters changed during stress treatments, and the change of specific extracellular resistance （r,） was the same as relaxation time （^-）. 4） The combination of the correlation analysis and the path analysis indicated that the soluble sugar content mainly impacted the re and a significant positive relation existed, and the starch content mainly impacted the distribution coefficient of relaxation time and a significant negative correlation was found during stress treatments. In conclusion, some of the EIS parameters could express effectively the changes of soluble sugar and starch contents in white birch roots under waterlogging and flooding stresses, and the EIS method is an effective way for the study of white birch roots＇ physiological attributes under adversity stress.

关 键 词：白桦 涝渍胁迫 电阻抗参数 可溶性糖 淀粉 

分 类 号：S718.45[农业科学—林学]