RNA干扰畸胎瘤源性生长因子基因对食管鳞癌细胞生物学行为的影响  

Effect of PC cell-derived growth factor RNA interference on biological behavior of esophageal squamous carcinoma cells

作　　者：郑庆丰[1] 柳硕岩[1] 王海燕[5] 王枫[1] 王镇[1] 陈啸风[1] 王健键[1] 应敏刚[1] 郑雄伟[2] 林贤东[2] 周智锋[3] 龚福生[4] 谢云青[4] 

机构地区：[1]福建医科大学教学医院福建省肿瘤医院胸外科福建省肿瘤生物治疗重点实验室,福州350014 [2]福建医科大学教学医院福建省肿瘤医院病理科,福州350014 [3]福建医科大学教学医院福建省肿瘤医院内科研究室,福州350014 [4]福建医科大学教学医院福建省肿瘤医院外科研究室,福州350014 [5]福建医科大学基础医学院病理学系

出　　处：《中华胃肠外科杂志》2013年第9期874-877,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：福建省卫生厅青年科研课题（2010-1-19）;国家自然科学基金青年科学基金（81201969）;福建省自然科学基金项目（2010J05054）

摘　　要：目的 探讨RNA干扰畸胎瘤源性生长因子（PCDGF）基因对食管鳞癌细胞Eca-109的影响.方法 脂质体介导PCDGF-shRNA的表达载体转染Eca-109细胞,应用RT-PCR、Westernblot检测转染后细胞PCDGF mRNA及蛋白的表达情况,分别采用Counting Kit-8（CCK-8）试剂盒和Boyden小室法检测转染后Eca-109细胞的增殖能力和侵袭能力.结果 转染组PCDGF mRNA和蛋白表达水平均较其他组下调.转染24、48和72 h转染组细胞增殖均受到抑制,抑制率分别为20.4％、21.1％和20.9％,其细胞增殖活性较未转染组、阴性质粒组和脂质体组均明显降低（均 P＜0.05）.未转染组、阴性质粒组、脂质体组和转染组的细胞迁移数分别为118.8±12.0、100.8±9.0、114.3±4.7和 53.5±16.3,转染组与其余3组比较,差异均有统计学意义（P=0.001,P=0.002,P=0.002）.结论 RNA干扰PCDGF基因可抑制食管鳞癌细胞的体外增殖及侵袭能力,PCDGF基因可能成为基因治疗的新靶点.Objective To investigate the effect of PC cell-derived growth factor （PCDGF） RNA interference on esophageal squamous carcinoma cells Eca-109 in vitro.Methods The PCDGF-shRNA expression vector was transfected into the Eca-109 cells by liposome.After transfection,the mRNA and protein expressions of PCDGF were detected by RT-PCR and Western-blot respectively.Cell Counting Kit-8 （CCK-8） assay and Boyden chamber method were performed to measure the cell proliferation and invasion ability respectively.Results The expression levels of PCDGF mRNA and protein were both decreased in Eca-109 cells transfected with PCDGF-shRNA expression vector （transfection group）.Twenty-four,48 and 72 h after transfection,the cells proliferation in the transfection group was inhibited,and the inhibition rate was 20.4%,21.1% and 20.9% respectively.The cell proliferation activity in the transfection group was significantly lower than that in the non-transfection group,liposome group and negative vector group （all P〈0.05）.The number of cell migration in the nontransfection group,negative vector group,liposome group and transfection group was 118.8 ±12.0,100.8±9.0,114.3 ±4.7,and 53.5±16.3 respectively.The differences were statistically significant between the transfection group and the other 3 groups （all P〈0.05）.Conclusions PCDGF RNA interference can inhibit the proliferation and invasion abilities of esophageal squamous carcinoma cells in vitro.PCDGF gene may be the new target of gene therapy.

关 键 词：食管肿瘤 畸胎瘤源性生长因子 RNA干扰 生物学特性 

分 类 号：R735.1[医药卫生—肿瘤]