慢性不可预测轻度应激诱导的抑郁模型大鼠行为学评定及血清谷胱甘肽过氧化物酶水平检测  

Ethology assessment and detection of serum GPX-3 levels in rat model of depression induced by chronic unpredictable mild stress

作　　者：朱长强[1] 李治国[1] 刘勇[1] 丁兴华[1] 杨树广[1] 刘少君[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850

出　　处：《解放军医学杂志》2013年第9期710-713,共4页Medical Journal of Chinese People's Liberation Army

基　　金：国家重点基础研究发展计划(973计划)资助项目(2009CB918301)~~

摘　　要：目的观察慢性不可预测轻度应激(CUMS)诱导的首发和复发抑郁模型大鼠的行为学改变以及血清谷胱甘肽过氧化物酶3(GPX-3)水平的变化。方法健康成年雄性SD大鼠24只,随机均分为4组:组1(正常饲养),组2(CUMS),组3(正常饲养+生理盐水+正常饲养),组4(CUMS+氟西汀+CUMS)。应用糖水消耗实验和旷野实验对各组大鼠进行行为学评定,采用酶联免疫吸附实验(ELISA)测定血清GPX-3含量。结果 4周CUMS后,与对照组(组1、组3)比较,实验组(组2、组4)大鼠糖水偏好度、活动能力均显著降低(P<0.01);经3周氟西汀治疗后(组4),大鼠的抑郁样行为发生逆转,与对照组(组3)相比无明显差异(P>0.05),治疗后大鼠再次给予4周CUMS后(组4),大鼠的糖水偏好度显著降低(P<0.01),活动路程与不动时间也低于对照组(组3,P<0.05)。首发和复发CUMS抑郁大鼠(组2和组4)血清GPX-3水平显著高于对照组(组1、组3,P<0.01)。结论 CUMS可诱导大鼠产生抑郁样症状,而氟西汀能逆转CUMS诱导的抑郁样症状;首发和复发CUMS抑郁大鼠血清GPX-3升高,可能有助于降低抑郁样大鼠体内氧化应激水平,从而对机体起到一定保护作用。Objective To investigate the ethological changes and detect the serum level of glutathione peroxidase（GPX3） in a rat model of primary and recurrent depression induced by chronic unpredictable mild stress（CUMS）.Methods Rats were divided into four groups： group 1（normal feeding）,group 2（CUMS）,group 3（normal ＋ saline ＋ normal feeding） and group 4（CUMS ＋ fluoxetine ＋ CUMS）.The behavior of rats in the four groups was assessed by sucrose preference test and open field test.GPX-3 concentration in serum was measured by ELISA.Results A four-week of CUMS was successful in inducing a significant decrease in sucrose preference and locomotor activity in rats in treatment groups（group 2 and 4） in contrast with that of control groups（group 1 and 3）（P0.01）.After 3 weeks of fluoxetine treatment,no obvious difference was found in rats between the group 4 and group 3 in the degree of sucrose preference and locomotor activity.Subsequently,after the treated rats were re-exposed to CUMS for 4 weeks,a significant difference was found again in sucrose preference test（P0.01） and open field test in comparison with control group（P0.05）.GPX-3 levels in the rats exposed and re-exposed to CUMS were significantly higher than those in control groups（P0.01）.Conclusions CUMS may induce depression-like symptoms in rats,and fluoxetine can alleviate the symptoms induced by CUMS.The increase in GPX-3 concentration may reduce the level of oxidative stress in depression-like rats exposed and re-exposed to CUMS,accordingly it plays a protective role in these rats.

关 键 词：抑郁 氟西汀 复发 慢性不可预测轻度应激 谷胱甘肽过氧化物酶 

分 类 号：R749.42[医药卫生—神经病学与精神病学]