RP-HPLC法测定美国不同产地野葛根和叶片中葛根素、大豆苷和大豆苷元含量的研究  

Determination of Puerarin,Daidzin and Daidzein in Roots and Leaves of Pueraria lobata(Wild) Ohwi from United States by RP-HPLC

作　　者：杨雪芳[1,2] 黄伟[1] 丁建清[1] 

机构地区：[1]中国科学院水生植物与流域生态重点实验室,中国科学院武汉植物园,武汉430074 [2]中国科学院大学,北京100049

出　　处：《植物科学学报》2013年第4期391-397,共7页Plant Science Journal

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-EW-J-20)

摘　　要：对采自美国的8个野葛种群的异黄酮类物质进行了定量分析。结果表明,叶片中葛根素、大豆苷和大豆苷元含量分别为0.0950、0.698、0.110 mg/g,但不同产地大豆苷和大豆苷元含量存在显著差异。在整个生长季节中,3种异黄酮类物质的含量均在8月份最高。根中3种异黄酮类物质的含量分别为3.664、1.0302、0.3689 mg/g,但不同产地之间无显著差异。此外,美国种群中异黄酮类物质的含量均显著高于已报道的中国种群。本研究为充分利用国内外野葛资源,确定合理采收时间,挖掘野葛叶片的利用潜力奠定了一定的理论基础,为葛藤新药源的开发利用提供了科学依据。In this study,we measured three isoflavonoids in kudzu from eight populations in south-eastern United States.The concentrations of puerarin,daidzin and daidzein in the leaves were 0.0950 mg/g,0.698 mg/g and 0.110 mg/g,respectively.The concentration of daidzin and daidzein was significantly different among populations.During the growing season,three isoflavonoids in the leaves were higher in August than in July and September.The concentrations of the three isoflavonoids in the roots were 3.664 mg/g,1.0302 mg/g and 0.3689 mg/g,respectively.There were no significant differences in the three isoflavonoids among populations.Furthermore,the concentrations of the three isoflavonoids in kudzu from US populations were higher than those of Chinese populations,as reported in previous research.This study aimed to provide a theoretic and scientific basis for making full use of kudzu leaves,determining appropriate harvest times and the development and utilization of the puerarin leaves resource.

关 键 词：野葛 异黄酮类化合物 资源收集和评价 

分 类 号：R282[医药卫生—中药学] R284.1[医药卫生—中医学]