放射性^(125)I组织间植入对裸鼠人胃癌细胞BGC-823移植瘤侵袭转移的影响  

Effects of Radioactive Seeds ^(125)I through Interstitial Implantation on Neoplasm Invasiveness in Xenograft Nude Mice Model of Human Gastric Carcinoma BGC-823

作　　者：宋波[1,2] 张万福[1] 李波[1] 毛文源[1] 罗开元[1] 

机构地区：[1]昆明医科大学第四附属医院普外科,昆明650021 [2]云南中医学院基础医学院

出　　处：《肿瘤防治研究》2013年第9期826-829,共4页Cancer Research on Prevention and Treatment

基　　金：云南省科技计划资助项目(2008CC002)

摘　　要：目的观察放射性125I组织间植入对人胃癌裸鼠移植瘤侵袭的抑制作用,并探讨其可能的作用机制。方法将人胃腺癌细胞株BGC-823接种BALB/C裸鼠右侧腋部皮下建立人胃癌裸鼠移植瘤模型后,随机分为模型对照组(对照组)以及放射治疗实验组(实验组)。对照组不接受125I组织间植入;实验组分别接受放射性125I组织间植入30d、60d和90d。通过测定肿瘤体质量、体积及肺转移结节观察其抑瘤作用,采用ELISA测定肿瘤端粒酶含量以及内皮生长因子(vascular endothelial growth factor,VEGF)和碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)含量,Western blot法检测磷酸化Erk(p-Erk)1/2蛋白表达。结果与对照组比较,放射性125I组织间植入30d、60d和90d后能够明显减少肿瘤体质量、体积及肺转移结节,降低肿瘤端粒酶以及内皮生长因子和成纤维细胞生长因子含量,同时抑制p-Erk1/2的表达(P<0.05或P<0.01),尤以125I植入90d效果最为显著。结论对Erk细胞信号转导通路的抑制可能是放射性125I近距离植入抑制人胃癌裸鼠移植瘤侵袭的机制之一。Objective To study the inhibitory effect of radioactive seeds ^125I through interstitial implantation on neoplasm invasiveness, and analyze possible mechanisms. Methods The BGC-823 cells were subcutaneously injected into the right anterior armpit of BALB/C nude mice in order to establish the model of metastatic tumor. The mice of metastatic tumor were randomly divided into control group and experimental group, and mice from the experimental group were treated by interstitial implantation of ^125I for 30, 60 and 90 days, respectively. The tumor inhibitory effect was detected by measuring tumor weigh, size and lung metastasis nodules. The levels of telomerase protein in metastatic tumor, VEGF and bFGF in serum were measured by ELISA, and the expression of p-Erk1/2 in tumor was analyzed by Western blot. Results Compared with the control group, tumor weight, size, lung metastasis nodules, and the levels of telomerase protein in metastatic tumor, VEGF and bFGF in serum were significantly decreased due to 1251 interstitially implanted. Meanwhile, the expression of p-Erk1/2 in tumor was down-regulated （P〈 0. 05 or P〈0. 01 ）, especially for 90-day treatment. Conclusion It could be the mechanism that Erk sig nal pathway in volved in neoplasm invasiveness is inhibited by radioactive seeds ^125I through interstitial implantation in xenograft nude mice of human gastric carcinoma.

关 键 词：放射性粒子 BGC-823 胃癌 ERK信号通路 抑制作用 

分 类 号：R735.2[医药卫生—肿瘤]