解毒三根汤干预肿瘤相关成纤维细胞及抑制结肠癌侵袭转移的实验研究  

Jiedu Sangen Decoction Intervened Carcinoma-Associated Fibroblasts and Inhibited Migration and Invasion of Colon Cancer:an Experimental Research

作　　者：阮善明[1] 沈敏鹤[1] 林红[2] 郑丽萍[2] 王益[2] 李梦婷[2] 

机构地区：[1]浙江中医药大学附属第一医院肿瘤科,杭州310006 [2]浙江中医药大学第一临床医学院,杭州310006

出　　处：《中国中西医结合杂志》2013年第9期1242-1246,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家公益性行业科研专项基金项目(No.200807018);浙江省教育厅科研项目(No.Y201016272);浙江省中医药优秀青年人才基金计划项目(No.2013ZQ011)

摘　　要：目的观察解毒三根汤(虎杖根、水杨梅根、藤梨根)对结肠癌CT-26细胞的迁移能力及对肿瘤相关成纤维细胞(CAFs)转化生长因子β1(transforming growth factor-β1,TGF-β1)、基质金属蛋白酶9(matrix metalloproteinase 9,MMP-9)、α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)的影响。方法 BALB/c小鼠皮下接种CT-26结肠癌细胞(1.2×106 mL);随机分为3组:正常对照组,模型组,解毒三根汤组。Transwell观察3组血清对CT-26细胞迁移力的影响,ELISA法检测3组CAFs上清液中TGF-β1、MMP-9的表达量,实时荧光定量PCR检测各组CAFs中α-SMA、TGF-β1mRNA表达量。结果解毒三根汤组透膜结肠癌CT-26细胞数量较模型组明显减少,差异有统计学意义(P<0.01);24h及48h解毒三根汤组刺激的CAFs上清液中TGF-β1、MMP-9的表达量较模型组降低(P<0.05,P<0.01);解毒三根汤组α-SMA、TGF-β1mRNA表达量较模型组明显降低,差异有统计学意义(P<0.01)。结论解毒三根汤降低CAFs上清液中TGF-β1、MMP-9的表达量,降低CAFs中α-SMA mRNA、TGF-β1mRNA的表达量,可能是其抑制肿瘤细胞迁移侵袭的机制。Objective To observe the effect of Jiedu Sangen Decoction （JSD, consisting of Polygo- num cuspidatum, Geurn Japonicum Thumnb, Radix Actinidiae Chinensis） on the migration capability of colon cancer CT-26 cells were observed, and on expressions of carcinoma-associated fibroblasts （CAFs） such as transforming growth factor-β1 （TGF-β1 ）, matrix metalloproteinase 9 （MMP-9）, and （x-smooth muscle actin （（x-SMA）. Methods The BALB/C mice were subcutaneously inoculated with colon cancer CT-26 cells （1.2 x 106mL） and then randomly divided into 3 groups, i.e., the normal control group, the model group, the JSD treated group. The effects of three different serums on the migration ability of colon cancer CT-26 cells were ob- served using Transwell. The expression quantities of TGF-β1 and MMP-9 in the supernatant of CAFs were detec- ted using ELISA. The mRNA expression quantities of TGF-β1 and α-SMA in CAFs were detected by real-time flu- orescence quantitative PCR. Results The number of semi-permeable film cells in the JSD treated group sig- nificantly decreased, when compared with the model group, showing statistical significance （P 〈0. 01 ）. Com- pared with the model group, the expressions of TGF-β1 and MMP-9 in the supernatant of CAFs decreased in the JSD treated group at 24 and 48 h, showing statistical difference （P 〈0.05, P 〈0.01 ）. Compared with the model group, the mRNA expressions of TGF-β1 and α-SMA in statistical difference （P 〈0.01 ）. Conclusion pernatant of CAFs, lower mRNA expressions JSD could of α-SMA the JSD treated group obviously decreased, showing decrease expressions of TGF-β1and MMP-9 in the su- and TGF--1, which might be possible mechanisms for inhibiting the migration and invasion of tumor cells.

关 键 词：清热解毒法 虎杖根 水杨梅根 藤梨根 CT-26结肠癌细胞 迁移 

分 类 号：R735.35[医药卫生—肿瘤] R285.5[医药卫生—临床医学]