鸭甲肝病毒3型2012年山东分离株VP1基因的序列分析  

Sequence Analysis of VP1 Gene of the Duck Hepatitis A Virus Type 3 Strains Isolated from Shandong Province of China in 2012

作　　者：徐倩[1,2] 陈琳琳[1,2] 张瑞华[1,2] 杨蕾[1,2] 谢之景[1,2] 朱岩丽[1,2] 姜世金[1,2] 司兴奎[3] 

机构地区：[1]山东农业大学动物医学院,泰安271018 [2]山东省动物生物工程与疾病防治重点实验室,泰安271018 [3]佛山科学技术学院动物医学系,佛山528231

出　　处：《病毒学报》2013年第5期522-528,共7页Chinese Journal of Virology

基　　金：国家自然科学基金项目(30871878);山东省高等学校科技计划项目(J08LF07);山东省科技发展计划项目(2010GNC10914)

摘　　要：为揭示近年来鸭甲肝病毒3型(DHAV-3)中国分离株VP1基因的遗传变异规律,本研究对2012年从山东省分离到的13株DHAV-3的VP1基因分别进行PCR扩增、序列测序与分析。结果显示,13株DHAV-3的VP1基因均由720个核苷酸组成,共编码240个氨基酸,核苷酸序列和氨基酸序列同源性分别为94.6%～99.9%和95.0%～100%。与GenBank中公布的31株DHAV-3的VP1基因的核苷酸和氨基酸序列同源性分别为92.5%～100%和90.8%～100%。系统进化分析显示,DHAV-3可分为两个基因型,其中除疫苗毒B63之外所有中国分离株均属于GⅠ型,越南分离毒株主要属于GⅡ型S1亚型,而韩国分离株组成GⅡ型中的S2亚型,具有明显的地域特征。To reveal the genetic variation of the viral protein 1 （VP1） gene of the duck hepatitis A virus type 3 （DHAV-3）, the VP1 gene of 13 virulent DHAV-3 strains isolated from Shandong province of China in 2012 were amplified by RT-PCR, sequenced and analyzed. The results showed that all the VP1 genes of the 13 isolates contained 720 nucleotides encoding 240 amino acids, and shared with nucleotide identities of 94.6%-99.9% and amino acid identities of 95.0%-100%. The nucleotide and amino acid sequence homolo- gies between the 13 DHAV-3 isolates and other 31 DHAV 3 reference strains were 92.5%-100% and 90.8%-100%, respectively. Phylogenetic analysis showed that the VP1 gene of DHAV-3 had distinct geo- graphical characteristics. Distribution of genotypes of the 44 DHAV-3 strains was as follows： except the vaccine strain B63, all the other Chinese isolates belonged to genotype I （G I ）, Vietnamese wild isolates mainly belonged to subtype 1 （S1） of genotype II （GII）, and all Korean isolates belonged to subtype 2 （S2） of GII.

关 键 词：DHAV-3 VP1基因 序列分析 GENBANK 

分 类 号：S852.65[农业科学—基础兽医学]