低强度聚焦超声联合载药微泡靶向治疗兔VX_2肿瘤的实验研究  

Treatment of Rabbit VX_2 Liver Cancer Using Drug-loaded Microbubbles and Low Intensity Focused Ultrasound-targeted Destruction

作　　者：董桂芳[1] 冉海涛[1] 王志刚[1] 宫玉萍[1] 王希[1] 

机构地区：[1]重庆医科大学附属第二医院超声影像学研究所,重庆市400010

出　　处：《中国超声医学杂志》2013年第9期836-839,共4页Chinese Journal of Ultrasound in Medicine

基　　金：重庆市科技攻关项目(CST C;2009AB5216);国家自然科学基金面上项目(No.81071157)

摘　　要：目的探讨自制低强度聚焦超声(low intensity focused ultrasound,LIFU)定位辐照载紫杉醇脂质微泡(pclitaxel-carrying liposome microbubble,PLM)靶向治疗兔VX_2肿瘤的治疗效果。方法建立兔VX_2肿瘤模型,20只荷瘤兔随机分为生理盐水对照组、单纯PLM组、非聚焦超声+PLM组、聚焦超声+PLM组。各组治疗前后均行超声检查观察肿瘤生长变化情况,比较各组肿瘤的抑瘤率。末次治疗结束后剥取肿瘤组织,TUNEL法检测细胞凋亡并进行半定量分析。结果聚焦超声+PLM组肿瘤体积增长最慢(P<0.05),抑瘤率、细胞凋亡指数明显高于其他各组(P<0.05),VX_2肿瘤的抑制作用最显著。结论低强度聚焦超声联合PLM能有效介导药物定位释放,进一步提高药物靶向性,有望成为专门化的用于药物体内定位控释的超声辐照系统。Objective To explore the treatment of rabbit VX2 tumor using Pclitaxel-carrying liposome microbub- ble（PLM） and low intensity focused ultrasound （LIFU） targeted destruction. Methods Prepare PLM. Set up rabbit VX2 liver tumor model. 20 rabbits were randomly divided into 4 groups： Control （A）, PLM （B）,Nowfocused ultra- sound ＋ PLM （C）, Focused ultrasound ＋PLM（D）. Observed the volumes of tumors before and after treatment with ultrasound , compared the tumor inhibition rate（TIR）. After all treatments, rabbits were sacrificed and the tumors were harvested to observe the apoptosis with TUNEL. Results In Group D, the growth of tumor volume was the slo- west , the TIR and AI were significantly higher than those of the other groups （P〈0. 05）, the effect of treatment was the best. Conclusions Drugs can be targeted-released using LIFU and drug-loaded microbubbles. The targeting of LI FU is improved further. LIFU is expected to be specialized ultrasonic irradiation system for positioning controlled re lease of drug in vivo.

关 键 词：低强度聚焦超声 PLM 肿瘤 靶向 

分 类 号：R73-36[医药卫生—肿瘤]