Preliminary molecular analysis of bacterial composition in periapical lesions with primary endodontic infections of deciduous teeth  

作　　者：SHANG Jia-jian YANG Qiu-bo ZHAO Huan-ying CAI Shuang ZHOU Yan SUN Zheng 

机构地区：[1]Capital Medical University School of Stomatology, Beijing 100050,China [2]Medical Experiment'and Test Center, Capital Medical University,Beijing 100069, China

出　　处：《Chinese Medical Journal》2013年第16期3112-3117,共6页中华医学杂志（英文版）

基　　金：This study was supported by the grants from the Beijing Natural Science Foundation （No. 7122079） and the Scientific Research Common Program of Beijing Municipal Commission of Education （No. SQKM 201210025019）.

摘　　要：Background The bacterial composition of periapical lesions in deciduous teeth has not been well documented.This study was designed to explore the bacterial compositions,especially the dominant bacteria in periapical lesions using 16S rRNA sequencing.Methods Tissue samples were collected from 11 periapical lesions in deciduous teeth with primary endodontic infections.DNA was extracted from each sample and analyzed using 16S rRNA cloning and sequencing for the identification of bacteria.Results All DNA samples were positive for 16S rRNA gene PCR.One hundred and fifty-one phylotypes from 810 clones were identified to eight phyla,and each sample contained an average of 25.9 phylotypes.In addition,59 phylotypes were detected in more than two samples,and Fusobacterium （F.） nucleatum （8/11）,Dialister （D.） invisus （8/11）,Campylobacter （C.） gracilis （7/11）,Escherichia （E.） coil DH1 （6/11）,Aggregatibacter （A.） segnis （6/11）,and Streptococcus （S.） mitis （6/11） were the most prevalent species.Furthermore,45 as-yet-uncultivated phylotypes were also identified.Conclusions Chronic periapical lesions in deciduous teeth contained polymicrobial infections.F.nucleatum,D.invisus,C.gracilis,E.coli DH1,A.segnis,and S.mitis were the most prevalent species detected by 16S rRNA sequencing.Background The bacterial composition of periapical lesions in deciduous teeth has not been well documented.This study was designed to explore the bacterial compositions,especially the dominant bacteria in periapical lesions using 16S rRNA sequencing.Methods Tissue samples were collected from 11 periapical lesions in deciduous teeth with primary endodontic infections.DNA was extracted from each sample and analyzed using 16S rRNA cloning and sequencing for the identification of bacteria.Results All DNA samples were positive for 16S rRNA gene PCR.One hundred and fifty-one phylotypes from 810 clones were identified to eight phyla,and each sample contained an average of 25.9 phylotypes.In addition,59 phylotypes were detected in more than two samples,and Fusobacterium （F.） nucleatum （8/11）,Dialister （D.） invisus （8/11）,Campylobacter （C.） gracilis （7/11）,Escherichia （E.） coil DH1 （6/11）,Aggregatibacter （A.） segnis （6/11）,and Streptococcus （S.） mitis （6/11） were the most prevalent species.Furthermore,45 as-yet-uncultivated phylotypes were also identified.Conclusions Chronic periapical lesions in deciduous teeth contained polymicrobial infections.F.nucleatum,D.invisus,C.gracilis,E.coli DH1,A.segnis,and S.mitis were the most prevalent species detected by 16S rRNA sequencing.

关 键 词：16S rRNA sequencing BACTERIA deciduous teeth periapical lesions 

分 类 号：R781.3[医药卫生—口腔医学]