Two closely neighboring hepatocellular carcinomas mimicking intrahepatic metastasis are confirmed as double primary tumors by the loss of heterozygosity analysis of microsatellites  

作　　者：ZHU Zhen ZHU Hong-fa GU Yi-jin CONG Wen-ming 

机构地区：[1]Department of Pathology, Eastern Hepatobiliary Surgery Hospital,Second Military Medical University, Shanghai 200438, China [2]Department of Pathology, Mount Sinai School of Medicine, OneGustave L. Levy Place, New York, NY 10029, USA

出　　处：《Chinese Medical Journal》2013年第16期3187-3189,共3页中华医学杂志（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China （Nos. 30872506 and 81072026）.

摘　　要：Hepatocellular carcinoma （HCC） is a common malignancy around the world.Among all HCCs,multifocal HCC accounts for 12%-19%.1 The origin of multifocal HCC can either be the intrahepatic metastasis of a single tumor clone,or independent primaries.2 It is crucial to distinguish intrahepatic metastatic tumor from multicentric HCC because this distinction has significant impact on tumor staging,treatment plan,and clinical outcome.Routine histological examination alone cannot determine the clonal origin of multifocal HCC and usually results in their arbitrary classification as satellite nodules.The only way to reliably determine clonality among multiple lesions is by molecular genetic analysis.We report a patient who underwent surgical resection for two neighboring hepatocellular carcinomas that were from independent primaries.These separate nodules mimicked a solitary primary with intrahepatic metastasis.Confirmation of separate clones was established by the loss of heterozygosity （LOH） of microsatellites.Hepatocellular carcinoma （HCC） is a common malignancy around the world.Among all HCCs,multifocal HCC accounts for 12%-19%.1 The origin of multifocal HCC can either be the intrahepatic metastasis of a single tumor clone,or independent primaries.2 It is crucial to distinguish intrahepatic metastatic tumor from multicentric HCC because this distinction has significant impact on tumor staging,treatment plan,and clinical outcome.Routine histological examination alone cannot determine the clonal origin of multifocal HCC and usually results in their arbitrary classification as satellite nodules.The only way to reliably determine clonality among multiple lesions is by molecular genetic analysis.We report a patient who underwent surgical resection for two neighboring hepatocellular carcinomas that were from independent primaries.These separate nodules mimicked a solitary primary with intrahepatic metastasis.Confirmation of separate clones was established by the loss of heterozygosity （LOH） of microsatellites.

关 键 词：hepatocellular carcinoma CLONALITY MICROSATELLITE loss ofheterozygosity 

分 类 号：R735.7[医药卫生—肿瘤]