米非司酮对早孕绒毛组织中MMP-26、TIMP-4表达的影响  

Effect of mifepristone on expression of MMP-26 and TIMP-4

作　　者：张爱娟[1] 修瑞杰[1] 侯自红[1] 岳秀英[2] 顾向应[1] 江昌新[1] 

机构地区：[1]天津医科大学总医院妇产科,天津300052 [2]天津市宝坻区人民医院妇产科,天津301800

出　　处：《生殖医学杂志》2013年第9期647-651,共5页Journal of Reproductive Medicine

摘　　要：目的研究米非司酮对早孕期绒毛组织中基质金属蛋白酶-26(MMP-26)、基质金属蛋白酶抑制剂-4(TIMP-4)表达的影响,探讨米非司酮终止早孕的机制。方法随机选择2011年7月~9月来天津医科大学总医院计划生育科就诊,自愿要求终止妊娠的正常早期妊娠(孕40~63d)妇女60例,分为米非司酮组和对照组(各30例),两组又进一步按照孕周分别分为A1和A2亚组(≤7孕周),B1和B2亚组(>7孕周),每组各15例。米非司酮组给予米非司酮150mg分次口服后行人工流产术;对照组仅行人工流产术。采集人工流产术后绒毛组织,常规固定、包埋,显微镜下观察绒毛微血管数量(VMs),免疫组织化学法检测MMP-26、TIMP-4在两组绒毛组织中的表达水平。结果米非司酮组和对照组光镜下VMs数量无显著性差异(P>0.05),但A组显著低于B组(P<0.05)。米非司酮组绒毛组织中MMP-26的表达显著低于对照组(P<0.05),且米非司酮A1组显著低于对照A2组(P<0.05),其余各亚组比较无显著性差异(P>0.05)。米非司酮组绒毛组织中TIMP-4的表达显著高于对照组(P<0.05),各亚组间比较无显著性差异(P>0.05)。结论米非司酮并未影响绒毛微血管生长,可能是由于此时血管正处于代偿期。米非司酮可能通过打破MMP-26和TIMP-4的平衡,这可能是导致流产的原因之一。Objective： To study the effect of mifepristone on the expression of matrix metalloproteinase-26 （MMP-26） and tissue inhibitors of metalloproteinases-4 （TIMP-4） in the villus of early pregnancy and explore the mechanism of mifepristone terminate early pregnancy. Methods： Sixty normal early pregnant （within 40-63 days） women who required to terminate pregnancy were selected in family planning outpatient of the General Hospital of Tianjin Medical University from July 2011 to September 2011. These women were divided into two groups （mifepristone group and control group） randomly. Each group had 30 patients and was further divided to subgroup AI＆A2 （47 weeks） and subgroups BI＆B2 （〉7 weeks）, 15 patients for each group. The patients in the mifepristone group were given 150 mg mifepristone before artificial abortion. The patients in the control group were only performed artificial abortion without medication. The villi were collected, fixed and embed routinely. The amount of villus microvessels （VMs） was evaluated under microscope and the expressions levels of MMP-26 and TIMP-4 in two groups were detected by immunohistochemistry method. Results： 1. There was no significant difference in the amount of VMs between the control group and the mifepristone group （P^0. 05） ,but there was significant difference between the group A and the group B （P^0.05）. 2. The expression level of MMP-26 in the villus was significantly lower in the mifepristone group than that in control group （P%0.05）. The expression level of MMP-26 in group A1 of mifepristone group was significantly lower than that in group A2 of control group as well （P〈0.05）. No significant difference was found between the subgroup B1 and B2.3. The expression level of TIMP-4 in mifepristone group was significantly higher than that in the control group （P〈0.05）. No significant difference was found between subgroup A1 and A2 or subgroup B1 and B2 （P〉0.05）. Conclusions： 1. There was no significant diff

关 键 词：基质金属蛋白酶-26 基质金属蛋白酶抑制剂-4 绒毛 米非司酮 

分 类 号：R[医药卫生]