灰兔眼巩膜厚度测量  

Scleral thickness topography of the adult Chinchilla pigmented rabbit with implications for transscleral drug delivery research

作　　者：李杰[1] 兰碧菲 赵春晖[1] 程凌云[1] 

机构地区：[1]温州医科大学眼科药物研究所,325027

出　　处：《中华眼视光学与视觉科学杂志》2013年第9期551-554,557,共5页Chinese Journal Of Optometry Ophthalmology And Visual Science

基　　金：国家自然科学基金（31271022）

摘　　要：目的测量灰兔不同部位巩膜厚度，了解巩膜部位与厚度变化关系，为经巩膜眼内给药实验研究提供诠释依据。方法实验研究。49只（98眼）成年青紫蓝灰兔经麻醉后，安乐液处死，立即摘去眼球．随后放人1-25％戊二醛和l％多聚甲醛固定液固定，制成石蜡切片后行常规苏木精一伊红（HE）染色。经生物显微镜观察拍照后用图像分析软件测量从角巩膜缘部到视神经部的巩膜厚度。对测量结果采用广义估计方程（GEE）及Tukev’sHSDPostHoeTest分析。结果下半部分兔眼巩膜比上半部分巩膜薄，分别是（342．9±91．3）μm和（400．4±67．6）μm（X2=43．57，P〈0．01，GEE）。上半部分巩膜厚度在赤道前后及后极部差异无统计学意义，平均为（366．8±56．3）μm。下半部分巩膜厚度在赤道前和赤道部为（340．9±72．5）μm和（340．8±76．3）μm，往后进一步变薄，赤道部后为（293．9±57．4）μm，后极部为（209．0±51．8）μm。上半部分和下半部分巩膜均在神经处最薄，分别为（273．5±90．5）μm和（187．7±60．1）μm，与其他部位相比，差异有统计学意义（P〈0．05，Tukey＇sHSDPostHoeTest）。结论兔眼巩膜上半部分比下半部分厚，从部位和厚度关系分析，厚度在角巩膜缘与赤道部之间变化较大，在赤道部和后极部之间变化较小。Objective To measure the scleral thickness of the rabbit eye at different locations in the globe, providing a theoretical basis for advanced research in transscleral drug delivery. Methods In this experimental study, 98 eyes of 49 adult pigmented rabbits were sacrificed after deep anesthesia and the eyeballs were enucleated and fixed in 1% buffered formaldehyde and 1.25% glutaraldehyde for a minimum of 36 hours. The eyes were processed for routine paraffin embedding and sectioning. The sagittal sections at the optic nerve level were collected and used for scleral thickness study. The images were acquired at serial locations between the limbus and optic nerve and the thickness was measured with SPOT image software. The data were analyzed with generalized estimating equations （GEE） and Tukey＇s HSD Post Hoc Test. Results In general, the mean scleral thickness of the inferior eye globe was significantly thinner than that of the superior globe （342.9±91.3 μm versus 400.4±67.6 μm, X2=43.57, P〈0.01, GEE）. There were no significant differences in the scleral thickness from the pre-equator vicinity to the posterior pole, with a mean thickness of 366.8±56.3μm at the superior globe. For the inferior globe, the mean scleral thickness at the pre-equator vicinity and at the equator was similar, 340.9±72.5 μm and 340.8±76.3μm. However, scleral thickness decreased to 293.9±57.4 μm at the post-equator vicinity and to 209.0±51.8 μm at the posterior pole. The thinnest sclera was located immediately around the optic nerve, 273.5±90.5μm for the superior and 187.7±60.1 μm for the inferior, compared to the other locations （P〈0.05, Tukey＇s HSD Post Hoc Test）. Conclusion Rabbit sclera is thinner than human sclera. In the rabbit eye globe, the superior sclera is significantly thicker than the inferior sclera and superior sclera is shorter and more uniform in thickness between the equator and the posterior pole. Scleral thickness has larger variations between the limbus and equator than the variations

关 键 词：兔 巩膜厚度 经巩膜给药 

分 类 号：R77[医药卫生—眼科]