p62蛋白的自身免疫反应及其在结肠癌中的表达研究  

Humoral autoimmune response of p62 and its tissue-specific expression in colon cancer

作　　者：刘卫红[1] 徐维家[1] 王波[1] 李志[1] 王青[1] 

机构地区：[1]大连市中心医院检验科,大连市116033

出　　处：《实用检验医师杂志》2013年第2期88-91,共4页Chinese Journal of Clinical Pathologist

摘　　要：目的探讨肿瘤相关抗原p62的自身免疫反应及其在结肠癌中的表达，评估其在结肠癌早期诊断、恶性程度及预后评估中的应用价值。方法收集64例结肠癌患者、42例结肠腺瘤患者和30例正常对照者的结肠组织及血清标本，采用免疫组化方法检测受检者p62蛋白的表达情况；用ELISA和western blotting方法对血清中p62抗体进行检测；血清抗核抗体（antinuclear antibody，ANA）采用间接免疫荧光方法检测。结果三组患者血清中p62抗体阳性率差异有统计学意义钟=0．291，B0．003），结肠癌患者血清p62抗体的阳性率为23．4％，明显高于结肠腺瘤组（4．8％）和正常对照组（2．9％），且差异均有统计学意义（P均〈0．01）；不同结肠组织中p62阳性率差异有统计学意义钟=0．695，P=0．000），64例结肠癌组织标本中有48例（75．0％）出现p62阳性表达，均高于结肠腺瘤组织（14.3％）和正常对照组织（0．0％），且差异均有统计学意义（P均〈0．01）；三组患者血清中ANA阳性率差异有统计学意义（r=0．629．P=0．000），ANA在结肠癌组中的阳性率为82．8％，明显高于结肠腺瘤组（21．4％）和正常对照组（17．6％），且差异均有统计学意义（P均〈0．01）。其中结肠癌组ANA阳性核型以胞核为主47．2％（25／53），其次是核仁型24．5％（13／53）和胞浆型28．3％（15／53），其核仁和胞浆核型比例均高于对照组和结肠腺瘤组。结论抗p62抗体和ANA的联合检测对于结肠癌的早期诊断、疾病预防和监测具有重要意义。Objective To understand the autoimmune response of p62 and its expression in colon cancer, and to evaluate its value in early diagnosis, malignant degree and prognostic evaluation of colon cancer. Methods 64 cases colon cancer patients, 42 cases colon adenomatous patients and 30 cases healthy controls were collected. The expression of p62 was detected by immunohistochemistry assay. The autoantibody to p62 in serum was evaluated by enzyme-linked immunosorbent assay and western blotting. The antinuclear antibody （ANA） was detected by indirect immunofluorescence method. Results There was statistical significance in the difference of p62 antibody positive rates among three groups （χ^2= 0.291 ,P= 0.003）. There was a relative higher frequency of p62 in colon cancer group （23.4%） compared to colon adenomatous group （4.8%） and normal control group （2.9%）, and the differences all had statistical significance （Pall〈 0.01 ）. There was statistical significance in the difference of p62 positive rates among different colon tissues （χ^2= 0.695,P= 0.000）. 64 cases colon cancer tissue specimens examined, 48 cases tissues （75.0%） expressed p62, which higher than colon adenomatous tissues （14.3%） and normal control tissues （0.0%）, and the differences all had statistical significance （Pall〈 0.01 ）. There was statistical significance in the difference of ANA positive rates among three groups （χ^2= 0.629 ,P= 0.000）. The positive rate of ANA in colon cancer group was 82.8%, which was significantly higher than that in colon adenomatous group （21.4% ） and normal control group （17.6%）, and the differences all had statistical significance （Pall〈 0.01 ）. The ANA positive karyotype in colon cancer group including nucelus karyotype accounts for 47.2% （25/53）, nucleolar pattern accounts for 24.5% （ 13/53 ）, and hyalomitome karyotype accounts for 28.3% （15/53）. The proportion of nucleolar pattern and hyalomitome karyotype in colon cancer tissues were all higher tha

关 键 词：自身免疫反应 P62 免疫诊断 结肠癌 

分 类 号：R735.8[医药卫生—肿瘤]