miR-140—3p在人肺腺癌A549细胞顺铂化疗敏感性中的作用研究  

Function of MiR-140-3p in Human Lung Adenocarcinoma Cell Line A549 Chemosensitivity to Cisplatin

作　　者：欧阳永日[1] 朱保义[2] 王希[1] 林芳[1] 陈曦[1] 任继鸿[1] 张惠中[1] 卫军霞[1] 董轲[1] 

机构地区：[1]第四军医大学唐都医院临床实验与检验、输血科,西安710038 [2]第四军医大学唐都医院眼科,西安710038

出　　处：《现代检验医学杂志》2013年第4期5-8,共4页Journal of Modern Laboratory Medicine

基　　金：基金项目：国家自然科学基金青年基金资助项目（项目编号：31100546）.

摘　　要：目的 探讨miR-140-3p与人肺腺癌A549细胞顺铂药物敏感性的关系.方法 化学合成miR-140-3p mimics及inhibitor,脂质体2000介导其转染入人肺腺癌A549细胞,利用荧光定量PCR方法观察转染细胞中miR-140-3p表达水平的变化;噻唑蓝（MTT）法检测A549细胞对顺铂作用的敏感性.结果 转染miR-140-3p mimics/inhibitor可分别升高（约30%）或降低（约30%）人肺腺癌A549细胞中miR-140-3p表达;同时,与对照组相比,转染miR-140-3p inhibitor的人肺腺癌A549细胞对顺铂药物作用的敏感度显著增加,细胞增殖速率降低33%（P〈0.05）;而转染miR-140-3p mimics的A549细胞对顺铂药物作用的敏感度显著降低,细胞增殖速率升高32%（P〈0.05）.结论 miR-140-3p的表达水平与人肺腺癌A549细胞对顺铂药物的敏感度呈负相关,提示miR-140-3p可能在A549细胞顺铂耐药过程中发挥重要作用.Objective To investigate the effect of microRNA-140-3p on cisplatin chemosensitivity in human lung adenocarci- noma eell line A549. Methods Chemosynthesis miR-140-3p inhibitor/mimics was transfected into A549 cells by Liposome 2000,the expression of miR-140-3p was measured by SYBR green real-time PCR. MTT assay was applied to indentify the chemosensitivity of A549 cells to cisplatin. Results The expressions of miR-140-3p were markly downregulated （about 30%） in miR-140-3p inhibitor transfected cells and up-regulated （about 30%） in miR-140-3p mimics transfected cells. Com- pared to negative control and miR-NC inhibitor transfectants,the rate of cell proliferation was nearly reduced to 67 %in miR- 140-3p inhibitor transfectants （P^0.05） ~otherwise, the rate of cell proliferation increased nearly 32 % （P〈0.05） in miR- 140-3p mimics transfectants. Conclusion The expression of miR-140-3p had negative correlation with the the chemosensitivity to CDDP of A549. Taken together,our findings indicated that miR-140-3p would play an important role in the CDDP-re- sistant of non-small cell lung cancer.

关 键 词：miR-140—3p 非小细胞肺癌 A549 顺铂 化疗敏感度 

分 类 号：R734.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]