口腔粘膜鳞癌中端粒酶活性的检测及意义  

Detection and effect of telomerase activity in oral mucosal squamous cell carcinoma

作　　者：姚华[1] 吴葆萱[2] 吴求亮[1] 张行[2] 

机构地区：[1]浙江大学医学院附属第一医院口腔科,杭州310003 [2]浙江大学医学院,杭州310003

出　　处：《中华口腔医学杂志》2000年第4期269-270,共2页Chinese Journal of Stomatology

摘　　要：目的　探讨端粒酶活化在口腔鳞癌发生、发展中的作用。方法　采用端粒重复扩增酶标法对 2 0例口腔鳞癌及 10例正常口腔粘膜 ,9例口腔鳞癌及其癌旁组织中的端粒酶活性定量检测。结果　口腔鳞癌中的端粒酶活性值明显高于正常口腔粘膜 ,9例自身对照癌组织的端粒酶活性值高于癌旁组织。结论　端粒酶的激活对口腔鳞癌的发生。Objective To study telomerase activity in 20 oral squamous cell carcinoma(SCC), and 10 normal oral tissues and 9 oral SCC and 9 adjacent normal tissue Methods A modified PCR based telomeric repeat amplification protocal assay(TRAP) was used Results The telomerase activity was significantly higher in SCC than that of normal mucosa ,and than that of adjacent tissue as well Conclusions These findings indicated that telomerase activation might be a critical step in the formation and development of cancer

关 键 词：端粒酶 口腔粘膜鳞癌 活性 TRAP酶标法 

分 类 号：R739.8[医药卫生—肿瘤]