恶臭假单胞菌发酵条件的研究  

Fermentation Conditions of Pseudomonas Putida against TMV

作　　者：郭丛[1,2] 杨金广[1] 翟熙伦[1,2] 宋丽云[1,2] 申莉莉[1] 钱玉梅[1] 黄瑾 王永[4] 李更新[5] 王凤龙[1] 

机构地区：[1]国家烟草专卖局烟草病虫害监测与综合治理重点开放实验室,中国农业科学院烟草研究所,青岛266101 [2]中国农业科学院研究生院,北京100081 [3]广西壮族自治区烟草公司百色市公司,广西百色533000 [4]山东中烟工业有限责任公司,济南250100 [5]山东潍坊烟草有限公司,山东潍坊261031

出　　处：《中国烟草科学》2013年第4期50-54,59,共6页Chinese Tobacco Science

基　　金：中国烟草总公司项目"全国烟草有害生物调查研究"(110200902065)

摘　　要：从未发病的K326烟株根际土壤中筛选出对TMV具有高度拮抗活性菌株A3，发酵液的抑制效果可达95％以上，为此，从碳源、氮源、pH、温度、通气量及发酵时间等方面对该菌株的摇瓶发酵条件进行研究。结果表明，A3菌株发酵适宜温度为28℃，在该温度下发酵60～70h达到菌体最大数量，且抑菌效价最高；以葡萄糖为适宜的碳源可以更好的促进菌量的生长和抗病毒物质的分泌，有机氮中酵母膏或蛋白胨对菌株的生长和抗病毒活性成分生成均能达到理想的效果，鱼粉则不能被A3菌株很好的利用；pH7是菌株最适宜的发酵范围，偏酸不利于菌株的生长；适当加大通气量能更好地促进A3菌株的繁殖及抗病毒物质的分泌。The bacterial strain A3 with high inhibition to TMV was isolated from healthy tobacco （K326） field. Its culture medium and fermentation conditions were investigated. The results showed that 28 ℃ was the most optimal fermentative temperature for A3 to grow and produce antiviral, and at optimal cultural temperature the amount and antiviral of A3 reached the highest level after 60-70 h respectively. Glucose was suitable carbon-source nutrition for the strain growth, and peptone or yeast was a suitable nitrogen source nutrition as well. The pH 7 was fit for A3 fermentation, and acidic condition was not favorable to the growth of strain A3. Ample air supplied markedly could promote growth and antiviral production of the strain A3.

关 键 词：烟草 TMV 恶臭假单胞菌菌株A3 发酵条件 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]