PRGD/PDLLA/β-TCP/NGF神经导管与骨髓间充质干细胞亲和性研究  

Research of PRGD/PDLLA/β-TCP/NGF Cellular Affinity on Bone Marrow Mesenchymal Stem Cells

作　　者：夏朝红[1,2] 李世普[2] 

机构地区：[1]武汉理工大学医院,湖北武汉430070 [2]武汉理工大学生物材料与工程研究中心,湖北武汉430070

出　　处：《武汉大学学报（理学版）》2013年第4期369-373,共5页Journal of Wuhan University:Natural Science Edition

基　　金：国家重点基础研究发展计划(973)项目(2011CB60620)资助

摘　　要：采用全骨髓分离法分离并纯化获得了大鼠骨髓间充质干细胞(BMMSCs),将复合材料PRGD/PDL-LA/β-TCP/NGF(精氨酸-甘氨酸-天冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/聚乳酸/β-磷酸三钙/神经生长因子)与大鼠骨髓间充质干细胞复合培养之后进行扫描电镜样品制备及观察,对其细胞亲和性进行评价.结果表明,PRGD/PDLLA/β-TCP/NGF复合材料与大鼠骨髓间充质干细胞具有良好的细胞亲和性.骨髓间充质干细胞在复合材料表面铺展良好,多数细胞呈现梭形,接近原代培养状态.The rat bone marrow mesenchymal stem cells（BMMSCs） were obtained from bone marrow and purified.BMMSCs were co-cultured with our synthesized composite： arginine-glycine-aspatic acid（RGD） peptide modification of poly {（lactic acid）-co-[（glycolic acid）-alt-（L-lysine）]}/poly（D,L-lactic acid）/β-tricalcium phosphate/nerve growth factor（PRGD/PDLLA/β-TCP/NGF）.Observed under scanning electron microscope,most BMMSCs showed spindle-shaped on the surface of PRGD/PDLLA/β-TCP/NGF composite,which is close to the primary culture condition of BMMSCs.The result indicates that PDLLA/PRGD/β-TCP/NGF composite has excellent cellular affinity and shows promising potentials in the fabrication of nerve tissue engineering scaffold.BMMSCs on PRGD/PDLLA/β-TCP/NGF composite exhibited good growth condition.

关 键 词：PRGD PDLLA Β-TCP NGF（精氨酸-甘氨酸-天冬氨酸多肽接枝聚（羟基乙酸-L-赖氨酸-乳酸） 聚乳酸 Β-磷酸三钙 神经生长因子）复合材料 大鼠骨髓间充质干细胞 原代培养 周围神经修复 细胞亲和性 

分 类 号：R318.08[医药卫生—生物医学工程]