射频消融联合激活的树突状细胞疫苗对新西兰大白兔肝脏原位种植VX2肿瘤治疗的实验研究  

Radiofrequency thermal abalation combined with activated dendritic cell vaccine treatment improve the prognosis of New Zealand white rabbit orthopedic implantation VX2 tumor model

作　　者：邱必军[1] 张会禄[1] 李萍[2] 夏强[1] 翟博[1] 

机构地区：[1]上海交通大学医学院附属仁济医院肝脏外科,上海200127 [2]上海交通大学医学院附属仁济医院超声诊断科

出　　处：《肝胆外科杂志》2013年第4期302-305,共4页Journal of Hepatobiliary Surgery

基　　金：国家自然基金(项目编号:81072027)

摘　　要：目的明确RFA联合激活的DC疫苗在对新西兰大白兔肝脏VX2种植瘤疗效以及生存率的影响。方法 24只雄性新西兰大白兔公分为2组(每组12只),分别为RFA+PBS以及RFA+DC组;建立新西兰大白兔肝脏VX2原位种植瘤模型,分离新西兰大白兔骨髓单核细胞体外培养并给予GM-CSF,IL-4诱导分化,加由VX2肿瘤组织制备的热休克抗原孵育加LPS刺激成熟,收集DC细胞接种至新西兰大白兔的爪垫处,3天后给予原位种植VX2肿瘤的新西兰大白兔进行超声引导下的射频消融治疗,观察90天后的存活率。结果 RFA对于肝脏原位种植VX2肿瘤的新西兰大白兔疗效确切,RFA联合激活的DC疫苗能够显著改善其生存率。Objective To investigate the effect and its impact on survival of RFA combined with activated DC vaccine treat- ment on the New Zealand white rabbit liver orthopedic implantation VX2 tumor model. Methods twenty-four male New Zealand white rabbits was divided to two groups（ 12 each group） ： RFA alone,and RFA combined with DC vaccine respectively;To establish the rabbit orthopedic implantation VX2 tumor model,isolation the bone marrow tissue of the New Zealand white rabbit, the bone marrow derived mononuclear cells cultures supplemented with hGM-CSF and hlL-4,heat shock antigens from VX2 tumor and LPS were supplemented for maturation of DC ,matured DC were used to inoculate the left limb claw pad of the rabbit,3 days later, all of the rabbits performed RFA treatment under the guide of color Doppler ultrasound, observing the survival rate from the time of establishment of the rabbit liver orthopedic VX2 tumor implantation model. Results The liver orthopedic VX2 tumor of New Zealand white rabbit could be effectively controlled through RFA treatment, RFA combined with activated DC vaccine could improve the prognosis significantly.

关 键 词：射频消融 新西兰大白兔原位种植VX2肿瘤模型 活化的树突状细胞疫苗预后 

分 类 号：R735.7[医药卫生—肿瘤]