奶牛布鲁氏菌的监测与分离鉴定  

Study on the Isolation and Identification of Brucella in Dairy Cows

作　　者：袁立岗[1] 易新萍[2] 蒲敬伟[1] 李岩[3] 石琴[1] 吕秀娟 

机构地区：[1]新疆生产建设兵团第十二师畜牧兽医工作站,乌鲁木齐830009 [2]新疆畜牧科学院兽医研究所,乌鲁木齐830000 [3]新疆生产建设兵团畜牧兽医工作总站,乌鲁木齐830063 [4]新疆维吾尔自治区兽药饲料监察所,乌鲁木齐830063

出　　处：《中国奶牛》2013年第15期38-40,共3页China Dairy Cattle

基　　金：国家星火计划项目:牛羊布鲁氏菌病防控技术应用与示范(2011GA891007);兵团重点领域科技攻关计划--集约化奶牛场繁殖障碍类传染病流行病学调查与防控技术研究(2013BA004)

摘　　要：本试验采用SAT方法对乌鲁木齐地区某牛场进行了布鲁氏菌病监测,对阳性奶牛的乳汁进行细菌分离培养,用VirB8-PCR方法对分离株VirB8基因进行扩增鉴定。结果表明,2个分离株均为布鲁氏菌,布鲁氏菌分子分型PCR的鉴定结果显示该牛场感染的自然菌株为布鲁氏菌牛种(3b,5,6,9型)。To investigate the prevalence of Brucella in Xinjiang, serum samples collected from 3 dairy farms were tested by serum agglutination test（SAT） for the detection of infected antibodies to Brucellosis. Two isolated strains which identified by VirB8-PCR were got from milk samples of positive cow by using serum agglutination test（SAT） of a cattle farm in Urumqi region. The results indicated that the isolated strains were Brucella. In addition to the AMOS-PCR assays, isolated strains of Brucella specifically were identified for B.abortus. Furthermore, it differentiated B. abortus biovars 3b,5,6 and 9.

关 键 词：牛乳 布鲁氏菌 分离鉴定 

分 类 号：S858.23[农业科学—临床兽医学]