Bi-PASA技术及其在RYR1基因多态性研究中的应用  

Bi PASA technique and its application in the study on the polymorphism of RYR1 gene

作　　者：吴彦宁[1] 刘红林[1] 陈杰[1] 徐银学[1] 姜志华[1] 

机构地区：[1]南京农业大学动物科技学院,南京210095

出　　处：《南京农业大学学报》2000年第2期69-71,共3页Journal of Nanjing Agricultural University

摘　　要：双向PCR扩增特异等位基因 (Bi PASA)是一项建立在PCR基础之上的分子生物学新技术。它通过 2对引物的一次PCR反应可以迅速、有效地检测出基因组中单个碱基的突变 ,从而识别个体基因型的差异。现以猪氟烷基因即兰尼定受体 (Ryanodinereceptor 1 )基因的多态性检测为例 ,介绍该技术的基本原理。Bidirectional PCR amplification of specific alleles(Bi PASA) is a simple method to distinguish single base changes and to genotype homozygotes and heterozygotes in a single PCR reaction.Two alleles are amplified simultaneously and in opposite directions relying on allele specific PCR.The technique requires two pairs of novel primers(two outer primers and two allele specific inner primers) and appropriate cycling conditions.The overview summarized the development and general protocols with an example of RYR1 gene.

关 键 词：Bi-PASA技术 RYR1基因多态性 杂合子 纯合子 

分 类 号：Q75[生物学—分子生物学] S813.1[农业科学—畜牧学]