以污泥提取液为生长介质的纤细角毛藻培养与CO_2生物固定  

Cultivation of Chaetoceros gracilis for the Bio-Fixation of CO_2 Using Extract of Sewage Sludge as Growth Medium

作　　者：孟范平[1] 李祥蕾[1] 谢爽[1] 于腾[1] 程凤莲[1] 

机构地区：[1]中国海洋大学海洋环境与生态教育部重点实验室,山东青岛266100

出　　处：《中国海洋大学学报（自然科学版）》2013年第9期89-95,共7页Periodical of Ocean University of China

基　　金：国家科技支撑计划课题项目(2011BAD14B04)资助

摘　　要：污水污泥(MSS)中含有大量的氮磷营养盐,以其替代传统培养基作为微藻的营养来源,将显著降低微藻吸收固定CO2的成本,增强微藻在工业碳减排中的应用潜力。本研究以污水污泥提取液与海水的混合液作为纤细角毛藻(Chaetoceros gracilis)的生长介质,同时通入高浓度(5%～20%)CO2,在优化培养条件的基础上,将微藻接种到螺旋管式光生物反应器中进行动态试验,并逐日测定相应的藻生物量和固碳能力。结果表明,在污泥提取液和海水的混合体系中,纤细角毛藻的最适生长条件为:通入10%CO2气体,污泥提取液和海水按照1∶29比例混合,温度为30℃,光照强度为6 000lx。当保持10%CO2的通入速度为20mL·min-1时,生物反应器的适宜运行条件为:藻接种量1×106cells·mL-1,循环流量1 200mL·h-1。在上述优化条件下,最大藻生物量产率(0.36g·L-1·d-1)和最高固碳速率(0.67g·L-1·d-1)出现在循环培养第5d;此外,培养液中氮、磷营养盐的利用程度较高,NO3--N、NH4+-N、NO2--N、PO43--P的去除率分别达到96.9%、93.3%、78.0%和88.5%。Nutrients in municipal sewage sludge （MSS） contains plenty of nitrogen and phosphorous. By using the extract of MSS instead of the traditional culture media as the nutrient source for the cultivation of marine microalgae, it will reduce the cost during bio-fixation of CO2 dramatically and make microalgae more attractive in the industrial carbon emission reduction. In this study, the cultivation conditions for mi- croalgae Chaetoceros gracilis, growing in the mixture of extract of MSS and seawater aerated by high con- centration of CO2, were optimized. Then, Chaetoceros gracilis inoculated in the mixture was recirculated in a helical photobioreactor, daily changes of algal biomass were determined and the carbon fixation rates were calculated. It showed that the optimal growth conditions for Chaetoceros gracilis in the mixed culture were as foltows： CO2 concentration at 10%, ratio of sludge extract fluid to seawater at 1 ： 29, temperature at 30℃ and light intensity at 6 000 Ix. In the photobioreactor, when 10% CO2 flow rate was maintained at 20 mL · min^-1 , high algal biomass was observed at inoculum cell density of 1×10^6 cells · mL^-1 and mixture circulation rate of I 200 mL · h-1. Under these conditions, the highest biomass productivity of 0. 36 g · L-1 · d-1 and carbon fixation rate of 0. 67 gCO2g · L-1 · d-1 were obtained after five days of cultivation with recirculation. In addition, the removal efficiency of NO3--N, NH4^＋ -N, NO2^- -N and PO4^3- -P reached 96.9%, 93. 3%, 78. 0% and 88. 5%, respectively.

关 键 词：纤细角毛藻 污泥提取液 光生物反应器 CO2 生物固定 

分 类 号：X171[环境科学与工程—环境科学]