SFE-CGC法测定腰痛片中主要成分的含量  

Determination of the major components in anti-lumbago tablets using SFE-CGC

作　　者：陈斌[1] 陈伟[2] 杨根金[1] 方慧生[1] 瞿振兴 吴玉田[1] 

机构地区：[1]第二军医大学药学院,上海200433 [2]福州医学高等专科学校,福建福州350003

出　　处：《中国药学杂志》2000年第10期696-698,共3页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目!( 3 9770 90 4)

摘　　要：目的　建立超临界流体萃取 (SFE)技术和毛细管气相色谱 (CGC)非在线联用技术 ,测定中成药腰痛片中的主要成分。方法　以SFE法进行样品前处理 ,CGC法采用含 5 %苯基硅氧烷交联毛细管柱 ,蒽作为内标进行定量。结果　对国内 5个厂家生产的腰痛片中的补骨脂素和异补骨脂素进行测定 ,含量分别为 :补骨脂素 0 .11～ 0 .5 1mg·g-1,异补骨脂素 0 .2 1～ 0 .5 8mg·g-1。结论　SFE CGC法是腰痛片质量控制的快速可靠、准确灵敏的方法。OBJECTIVE To establish a method based on the off line coupling of SFE and CGC for quantitative analysis of the major components in anti lumbago tablets.METHODS Samples prepared by SFE were analyzed upon following conditions:crosslinked 5% Ph Me Silicone as stationary phase;FID detector,anthracene as the internal standard.RESULTS Several samples from 5 factories were determinated by this method.The content of psoralen ranged from 0.11～0.51 mg·g -1 .The content of isopsoralen varied from 0.21～0.58 mg·g -1 .CONCLUSION SFE as an alternative technique for sample preparation will be useful in the quality control of Chinese patent medicines.

关 键 词：超临界流体萃取 腰痛片 补骨脂素 异补骨脂素 

分 类 号：R286.0[医药卫生—中药学]