胶原酶在大鼠颈动脉肌内膜增殖模型中的应用  

Application of collagenase endothelial denudation in rat carotid artery myointimal hyperplasia model

作　　者：郗永安[1] 汪丽蕙[2] 唐朝枢[2] 张钧华[2] 朱国英[2] 

机构地区：[1]中国医学科学院北京天坛医院CCU,100050 [2]北京大学第一医院心血管研究所

出　　处：《中华胸心血管外科杂志》2000年第5期289-290,共2页Chinese Journal of Thoracic and Cardiovascular Surgery

摘　　要：目的　动态观察胶原酶消化法内皮剥脱对大鼠颈动脉肌内膜增殖的影响及形态学特征 ,探讨增殖性血管疾病的新模型。方法　 6 6只雄性SD大鼠随机分组 ,局部胶原酶消化 ,分时段取材制片观察。结果　术后即刻内皮完全剥脱 ,内弹力层部分破坏。术后 7d新生内皮重新覆盖腔面 ,中膜平滑肌细胞表型改变 ,向内膜迁移、异地增殖、合成分泌细胞外基质 ,内膜进行性增厚 ;14d达高峰 ,管腔明显狭窄。结论　胶原酶消化法内皮剥脱完全 ,血管肌内膜增殖显著 。Objective To study the morphology of myointimal hyperplasia following collagenase endothelial denudation in rat carotid artery at a new model for proliferative vascular diseases. Methods Sixty six SD rats were randomized into experimental and control groups. Animals in experimental group were treated with the enzymes and then sacrificed at different(range from 0 to 21 days). The vessels were excised and processed for histologic and morphologic analysis. Results Immediately after collagenase digestion the endothelium was completely denuded and the internal elastic lamina was partly damaged. After 7 days the endothelium recovered in most part of the injured area. The smooth muscle cells in media alter in their phenotypes, migrate to and proliferate in the intima, where they synthesized and secreted a large amount of extra cellular matrix substance forming progressive intimal thickening. The hyperplasia reached its maximum on 21 days after injury, leading to a significant stenosis within the artery. Conclusion The myointimal hyperplasia can be steadly produced by the method with little damage to media and the degree of injury to the artery wall can be controlled. The new model is suitable for studying the relationship between endothelial injury and proliferative vessel diseases.

关 键 词：胶原酶 血管疾病 肌内膜增殖 动脉粥样硬化 

分 类 号：R363[医药卫生—病理学]