SYT-SSX1和SYT-SSX2基因在滑膜肉瘤中的表达及功能  

Expression and function evaluation of SYT-SSX1 and SYT-SSX2 genes in synovial sarcoma

作　　者：任婷婷[1] 卢群山[1] 李大森[1] 郭卫[1] 

机构地区：[1]北京大学人民医院骨与软组织肿瘤治疗中心,100044

出　　处：《肿瘤研究与临床》2013年第8期505-508,共4页Cancer Research and Clinic

基　　金：国家自然科学基金（81001193）;教育部博士点新教师基金（20100001120077）

摘　　要：目的 研究融合基因SYT-SSX1和SYT-SSX2在滑膜肉瘤中的表达及功能。方法 收集原发滑膜肉瘤标本22例及患者临床资料，采用反转录聚合酶链反应法检测SYT-SSX1和SYT-SSX2的表达并统计分析其与病理因素的相关性；应用脂质体将携带SYT-SSX1和SYT-SSX2基因的重组质粒转染到小鼠胚胎成纤维细胞系NIH3T3，四甲基偶氮唑蓝（MTT）法和迁移侵袭实验检测SYT-SSX1和SYT-SSX2的作用。结果 22例标本中11例表达SYT-SSX1，9例表达SYT-SSX2，2例两种基因均未检测到。融合基因的比例大约为1∶1，SYT-SSX1融合基因多见于在双向性滑膜肉瘤且SYT-SSX2阳性患者的肿瘤体积明显小于SYT-SSX1阳性患者的肿瘤体积（以5 cm为分隔）（P＝0.028）。稳定表达SYT-SSX1和表达SYT-SSX2的NIH3T3细胞的增殖、迁移和侵袭能力都明显增强，且SYT-SSX1阳性细胞强于SYT-SSX2阳性细胞。结论 SYT-SSX1和SYT-SSX2在滑膜肉瘤中的表达比例约为1∶1，SYT-SSX1及SYT-SSX2均能促进细胞的生长和转移，且SYT-SSX1的恶性表型更高。Objective To investigate the expressions and functions of the fusion gene SYT-SSX1 and SYT-SSX2 in synovial sarcoma. Methods The synovial sarcoma tissue samples and clinical data of 22 synovial sarcoma patients were collected. The expressions of fusion genes were detected by RT-PCR. The relationships between fusion gene and clinicopathologic factors were statistically analyzed. The abilities of proliferation, migration and invasion of 3T3 cells transfected with fusion gene plasmids were detected by MTT, migration and invasion assays. Results There were 11 cases with expression of SYT-SSX1 gene and 9 cases with that of SYT-SSX2 genes. The ratio of SYT-SSX1 and SYT-SSX2 was about 1∶1. The SYT-SSX1 positive tumors were most biphasic SS and the tumor volumes of patients with SYT-SSX1 positive were larger than that of patients with SYT-SSX2 positive （P = 0.028）. The SYT-SSX1 positive NIH3T3 cells exhibited higher abilities of proliferation, migration and invasion than SYT-SSX2 positive. Conclusions The ratio of expression of SYT-SSX1 and SYT-SSX2 in synovial sarcoma was about 1∶1. Both SYT-SSX1 and SYT-SSX2 can promote the growth and migration in NIH3T3 cell. The abilities of proliferation and migration of SYT-SS1 were more potent than that of SYT-SSX2.

关 键 词：肉瘤 滑膜 SYT-SSX1 SYT-SSX2 细胞增殖 肿瘤转移 

分 类 号：R738.5[医药卫生—肿瘤]