宫颈鳞癌组织中MMP-9和VEGF的表达与微血管密度相关性研究  

Study of the relationship of the expression of MMP-9 and VEGF with microvascular density in squamous carcinoma of cervix

作　　者：杜晓燕 孔卫平[2] 张景丽 李毓琴 杨爱荣 陈彦亮 卢红 

机构地区：[1]河北省涉县肿瘤医院,河北涉县056404 [2]河北省邯郸市妇幼保健院,河北邯郸056002

出　　处：《现代中西医结合杂志》2013年第26期2856-2857,2860,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的探讨金属蛋白酶-9(MMP-9)和血管内皮生长因子(VEGF)在宫颈上皮内瘤样病变(CIN)和宫颈鳞癌中的表达及其相关性。方法选择手术切除的宫颈鳞癌组织标本47份作为肿瘤组,宫颈上皮内瘤样病变(CIN)标本35份作为CIN组,正常宫颈组织(NCE)21份作为NCE组,采用免疫组化SP法检测各组MMP-9和VECF的表达和微血管密度(MVD)(CD34标记)。结果肿瘤组、CIN组中MMP-9和VEGF阳性率和MVD值均明显高于NCE组(P<0.05),肿瘤组明显高于CIN组(P<0.05);MMP-9和VEGF阳性表达均与肿瘤组的临床分期、组织分化程度、有无淋巴结转移相关,并且在宫颈鳞癌组织中两者表达呈正相关;肿瘤组中MMP-9和VEG;F阳性者的MVD值明显高于其阴性者(P<0.05),MMP-9和VEGF的表达与MVD值存在显著性差异(P<0.05)。结论 MMP-9和VEGF在宫颈鳞癌发生、发展中可能起重要作用,MMP-9和VEGF及MVD值与宫颈鳞癌的浸润转移密切相关,可作为预后的判断指标。Objective It is to explore the expression of MMP -9 and VEGF in CIN and squamous carcinoma of cervix and its relationship. Methods The expression of MMP -9 and VEGF, microvessel density（MVD） labeled by CD43 in 21 cases of normal cervical epithelium （NCE） （ NCE group）, 35 cases of cervical intraepithelial neoplasm （ CIN ） （ CIN group ） and 47cases of squamous carcinoma of cervix （tumor group） were detected by immunohistochemistry SP method. Results The positive ex- pression rates of MMP - 9 and VEGF and MVD in tumor group and CIN group were significantly higher than that in NCE group （ P 〈 0. 05 ）. The positive expression rates in tumor group was were higher than that in CIN group（ P 〈0. 05 ） Both of their ex- pressions were related with lymph node metastasis, clinical stage and pathological staging（ P 〈 0.05 ）. MVD of the patients with positive MMP - 9 and VEGF was obviously higher than that with negative MMP - 9 and VEGF in tumor patients （ P 〈 0.05 ） , there was significant difference in MVD And the expressions in squamous carcinoma were positively related with each other. The expressions of MMP -9 and VEGF were positively correlated with MVD （P 〈 0.05 ）. Conclusion The positive ex- pression of MMP - 9 and VEGF may play an important role during the development of tumor, MMP - 9 and VEGF and MVD are closely related with the invasion and metastasis of tumor, which can be used as the indexes for the prognosis of tumor.

关 键 词：子宫内膜肿瘤 金属蛋白酶-9 血管内皮生长因子 免疫组织化学 微血管密度 

分 类 号：R737.33[医药卫生—肿瘤]