基于同源模建方法对黄地老虎信息素结合蛋白三维结构的预测与分析  

Homology Modeling and AnalyzationofPheromone Binding Protein of Agrotis segetum

作　　者：孙浩[1] 赵雷修[1] 周凡蕊[1] 高慧[2] 

机构地区：[1]西南林业大学理学院,云南昆明650224 [2]云南中医学院,云南昆明650500

出　　处：《农业灾害研究》2013年第6期11-14,共4页Journal of Agricultural Catastrophology

基　　金：西南林业大学重点科研基金项目(110932)

摘　　要：[目的]对黄地老虎Agrotis segetum信息素结合蛋白的三维结构进行预测和分析。[方法]用同源模建的方法构建了黄地老虎信息素结合蛋白的三维结构。通过Procheck、Verify_3D、Errat和ProSa2003等程序的评价,表明模建结构是可靠的。[结果]模建结构显示黄地老虎的信息素结合蛋白由6个α-螺旋和连接这些螺旋的回折构成,底物结合口袋成锥形。6个保守的半胱氨酸形成3个对结构起稳定作用的二硫键。[结论]预测结构可靠,为黄地老虎的研究奠定了理论基础。[Objecticve] The study aimed to predict the three dimension structure of pheromone binding protein of Agrotis segetura. [ Method ] The three dimension structure of pheromone binding protein of Agrotis segetum was modeled by ho- mology modeling method. The evalua- tion of the model by Procheck, Veri- fy_3D, Errat and ProSa2003 indicated that the model was reliable. [ Result] The pheromone binding protein of Agrotis segetura consists of 6 a-helices and loops between them. 6 conserved cys- teines form 3 disulfide bonds which could stabilize the structure. [Conclusion] The prediction structure was reliable. This re- search could provide a foundation for A - grotis segetum research.

关 键 词：信息素结合蛋白 黄地老虎 同源模建 

分 类 号：Q556[生物学—生物化学]