独一味4种药用成分含量的测定  

Simultaneous Determination of Four Medicinal Components in Lamiophlomis rotate

作　　者：何映霞[1] 张来[1] 沈昱翔[1] 龙菊[1] 

机构地区：[1]安顺学院化学与生物农学系,贵州省教育厅功能材料与资源化学特色重点实验室,贵州安顺561000

出　　处：《贵州农业科学》2013年第8期73-75,共3页Guizhou Agricultural Sciences

基　　金：贵州省中药现代化专项[黔科合中药字(2011)5053]

摘　　要：为了建立同时测定独一味中连翘酯苷B、毛蕊花糖苷、木犀草苷和木犀草素4种药用成分含量的高效液相色谱（HPLC）检测方法,采用独一味样品的甲醇提取物,建立了该测定方法,并进行了连翘酯苷B、毛蕊花糖苷、木犀草苷和木犀草素的线性范围和平均加样回收率的分析.结果表明：独一味样品中含连翘酯苷B、毛蕊花糖苷、木犀草苷和木犀草素4种药用成分；精密度,连翘酯苷B、毛蕊花糖苷、木犀草苷和木犀草素的峰面积（RSD）分别为0.65％、0.92％、0.73％和0.77％；稳定性,连翘酯苷B、毛蕊花糖苷、木犀草苷和木犀草素的RSD分别为0.78％、1.15％、1.06％和1.22％；重复性,连翘酯苷B、毛蕊花糖苷、木犀草苷和木犀草素的RSD分别为1.35％、0.76％、1.18％和0.91％；4种药用成分的加样回收率分别为100.66％、99.32％、97.07％和99.65％,RSD分别为2.83％、1.97％、2.01％和1.58％;10批药材中4种药用成分的含量分别为0.430～6.782mg/g、0.661～8.600 mg/g、1.320～6.877 mg/g和0.043～0.154mg/g.该方法准确可靠,重复性好,可用于独一味中连翘酯苷B、毛蕊花糖苷、木犀草苷和木犀草素含量的同时测定.To establish a HPLC method for determination of forsythoside B,acteoside,luteoloside and luteolin in L.rotate,the determination method was established by using methanol extracts from L.rotate sample,the linearity range and average recovery rate of them were analyzed.The results showed that four medicinal components,forsythoside B,acteoside,luteoloside and luteolin,were obtained in L.rotate sample; Degree of precision,RSD of the four medicinal components was 0.65%,0.92%,0.73% and 0.77% respectively.Stability,RSD was 0.78%,1.15%,1.06% and 1.22% separately;RSD was 1.35%,0.76%,1.18% and 0.91% respectively.The recovery rates of the four medicinal components were 100.66%,99.32%,97.07% and 99.65% with RSD of 2.83%,1.97%,2.01% and 1.58% respectively.The contents of the four medicinal components of the ten groups of medicinal materials were respectively 0.430～6.782 mg/g,0.661～8.600 mg/g,1.320～6.877 mg/g and 0.043～0.154 mg/g respectively.This method could be used for simultaneous determination of forsythoside B,acteoside,luteoloside and luteolin in L.rotate for its accuracy,reliability and good repeatability.

关 键 词：独一味 连翘酯苷B 毛蕊花糖苷 木犀草苷 木犀草素 HPLC 

分 类 号：S567[农业科学—中草药栽培]